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pubmed-article:588578pubmed:abstractTextStrain L mouse fibroblasts grown in medium supplemented with 2.5% delipidized horse serum were found capable of desaturating oleic and linoleic acid to dienoic and trienoic acid(s), respectively. Although 40-60% of de novo fatty acid synthesis from [2-3H]acetate was inhibited by the administration of exogenous oleic or linoleic acid, sterole synthesis was only slightly affected. Within 24-48 h after incorporation, phospholipid fatty acyl groups could undergo active exchange between phospholipids. After this dynamic transition period was over, not only were the phospholipid acyls retained but some vicinal fatty acyl pairs of phospholipid also appeared to be stable and remained together throughout the depletion period. At any time in the experiment, however, introduction of exogenous fatty acid perturbed this phospholipid acyl retention, delayed the time at which the phospholipid acyl groups no longer moved between phospholipids and also decreased the ultimate number of phospholipid acyl groups retained by strain L mouse fibroblasts.lld:pubmed
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pubmed-article:588578pubmed:articleTitleFatty acid synthesis and metabolism of phospholipid acyl groups in strain L mouse fibroblasts.lld:pubmed
pubmed-article:588578pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:588578pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed