| pubmed-article:4722901 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4722901 | lifeskim:mentions | umls-concept:C0205103 | lld:lifeskim |
| pubmed-article:4722901 | lifeskim:mentions | umls-concept:C1158366 | lld:lifeskim |
| pubmed-article:4722901 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:4722901 | pubmed:dateCreated | 1973-10-9 | lld:pubmed |
| pubmed-article:4722901 | pubmed:abstractText | 1. Aqueous extracts of acetone-dried liver and kidney mitochondria, supplemented with NAD(+), CoA and phenazine methosulphate, efficiently convert fatty-acyl-CoA compounds into acetyl-CoA; the process was followed with an O(2) electrode. 2. Label from [1-(14)C]octanoyl-CoA appears in acetyl-CoA more rapidly than that from [8-(14)C]octanoyl-CoA. 3. Oxidation of [8-(14)C]octanoyl-CoA was terminated by addition of neutral ethanolic hydroxylamine and the resulting hydroxamates were separated chromatographically. Hydroxamate derivatives of 3-hydroxyoctanoyl-, hexanoyl-, butyryl- and acetyl-CoA were obtained. 4. These and other observations suggest that oxidation of octanoyl-CoA by extracts involves participation of free intermediates rather than uninterrupted complete degradation of individual molecules to acetyl-CoA by a multienzyme complex. 5. Intact liver mitochondria studied by the hydroxamate technique were also shown to form intermediates during oxidation of labelled octanoates. In addition to octanoylhydroxamate, [8-(14)C]octanoate gave rise to small amounts of hexanoyl-, butyryl- and 3-hydroxyoctanoyl-hydroxamate. In contrast with extracts, however, where the quantity of intermediates found was a significant fraction of the precursors, mitochondria oxidizing octanoate contained much larger quantities of octanoyl-CoA than of any other intermediate. | lld:pubmed |
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| pubmed-article:4722901 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4722901 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4722901 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4722901 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4722901 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:4722901 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:4722901 | pubmed:author | pubmed-author:TubbsP KPK | lld:pubmed |
| pubmed-article:4722901 | pubmed:author | pubmed-author:StewartH BHB | lld:pubmed |
| pubmed-article:4722901 | pubmed:author | pubmed-author:StanleyK KKK | lld:pubmed |
| pubmed-article:4722901 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4722901 | pubmed:volume | 132 | lld:pubmed |
| pubmed-article:4722901 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4722901 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4722901 | pubmed:pagination | 61-76 | lld:pubmed |
| pubmed-article:4722901 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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| pubmed-article:4722901 | pubmed:year | 1973 | lld:pubmed |
| pubmed-article:4722901 | pubmed:articleTitle | Intermediates in fatty acid oxidation. | lld:pubmed |
| pubmed-article:4722901 | pubmed:publicationType | Journal Article | lld:pubmed |
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