| pubmed-article:444648 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:444648 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:444648 | lifeskim:mentions | umls-concept:C1621976 | lld:lifeskim |
| pubmed-article:444648 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:444648 | lifeskim:mentions | umls-concept:C2700400 | lld:lifeskim |
| pubmed-article:444648 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:444648 | pubmed:dateCreated | 1979-8-29 | lld:pubmed |
| pubmed-article:444648 | pubmed:abstractText | Platelets contain a polypeptide growth factor that stimulates the replication of normal connective tissue cells; this platelet-derived growth factor (PDGF) is released during the clotting process. Human platelets from normal volunteers were disrupted by nitrogen cavitation, and the subcellular organelles were fractionated by ultracentrifugation through a 30%--60% sucrose gradient. Electron microscopy revealed that fraction 7 (density 1.23 g/liter) contained the largest number of alpha granules. The specific activity of platelet fibrinogen, an alpha-granule marker, was also highest in this fraction. The subcellular fractions were assay for the presence of PDGF and for beta-thromboglobulin. PDGF was assayed quantitatively by the stimulation of DNA synthesis in confluent growth-arrested BALB/c-3T3 cells, whereas the concentration of beta-thromboglobulin was determined by radioimmunoassay. The highest concentrations of both PDGF and beta-thromboglobulin were found in the alpha-granule fraction. In contrast, beta-glucuronidase, a lysosomal enzyme, was more diffusely distributed and had its highest specific activity in fractions of lower density than those for PDGS, beta-thromboglobulin, or fibrinogen. The data demonstrate that the alpha granules of platelets provide a unique delivery system for PDGF, a polypeptide hormone with growth-promoting activity for connective tissue cells. | lld:pubmed |
| pubmed-article:444648 | pubmed:language | eng | lld:pubmed |
| pubmed-article:444648 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:444648 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:444648 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:444648 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:444648 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:444648 | pubmed:author | pubmed-author:StilesC DCD | lld:pubmed |
| pubmed-article:444648 | pubmed:author | pubmed-author:ChaoF CFC | lld:pubmed |
| pubmed-article:444648 | pubmed:author | pubmed-author:AntoniadesH... | lld:pubmed |
| pubmed-article:444648 | pubmed:author | pubmed-author:ScherC DCD | lld:pubmed |
| pubmed-article:444648 | pubmed:author | pubmed-author:KaplanD RDR | lld:pubmed |
| pubmed-article:444648 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:444648 | pubmed:volume | 53 | lld:pubmed |
| pubmed-article:444648 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:444648 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:444648 | pubmed:pagination | 1043-52 | lld:pubmed |
| pubmed-article:444648 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:444648 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:444648 | pubmed:articleTitle | Platelet alpha granules contain a growth factor for fibroblasts. | lld:pubmed |
| pubmed-article:444648 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:444648 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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