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pubmed-article:40719pubmed:abstractTextA continuous flow method has been developed for the automatic determination of enterokinase in rat small intesstine mucosa and/or luminal content. Trypsinogen was first hydrolysed by enterokinase under conditions which minimize autocatalytic activation. L-benzoyl-arginine paranitroanilide was then added and split to paranitroaniline by the trypsin so formed. Liberated paranitroalinine was diazotized and converted by the Bratton-Marshall reagent (N-naphthyl ethylene diamine) to an azodye, with maximum absorption at 550 nm. This method of determination was found to be six times more sensitive than the direct p-nitroaniline determination method. 36 determinations can be made hourly.lld:pubmed
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pubmed-article:40719pubmed:pagination187-194lld:pubmed
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pubmed-article:40719pubmed:articleTitleAn automated continuous flow procedure for the determination of enterokinase.lld:pubmed
pubmed-article:40719pubmed:publicationTypeJournal Articlelld:pubmed