pubmed-article:406901 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0237868 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0036679 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0002515 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C2828366 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C1705851 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C1555707 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
pubmed-article:406901 | lifeskim:mentions | umls-concept:C2752151 | lld:lifeskim |
pubmed-article:406901 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:406901 | pubmed:dateCreated | 1977-8-12 | lld:pubmed |
pubmed-article:406901 | pubmed:abstractText | Two main arylamidase activities were separated from a particle-free supernatant of rat heart by chromatography on DEAE-Sephadex. Although both enzymes hydrolysed L-leucine 4-nitroanilide, only peak-II enzyme hydrolysed L-lysine 4-nitroanilide. A third minor peak (Ia) contained an enzyme that was active mainly on the L-lysine 4-nitroanilide. The mol.wts. of the enzymes in peaks I and II were approx. 257000 and 105000 respectively. The pH optimum was approx. pH7.0 for peak-I enzyme and 7.0-8.0 for peak-II enzyme. Both enzymes were inhibited by addition of puromycin, p-hydroxymercuribenzoate, o-phenanthroline and bivalent metal ions. Addition of dithiothreitol resulted in stimulation of both activities. Dialysis against o-phenanthroline resulted in inhibition of peak-I and -II enzymes, but after dialysis against EDTA only peak-II enzyme was inhibited. | lld:pubmed |
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pubmed-article:406901 | pubmed:language | eng | lld:pubmed |
pubmed-article:406901 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:406901 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:406901 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:406901 | pubmed:month | Jun | lld:pubmed |
pubmed-article:406901 | pubmed:issn | 0264-6021 | lld:pubmed |
pubmed-article:406901 | pubmed:author | pubmed-author:BuryA FAF | lld:pubmed |
pubmed-article:406901 | pubmed:author | pubmed-author:CoolbearTT | lld:pubmed |
pubmed-article:406901 | pubmed:author | pubmed-author:SaveryC RCR | lld:pubmed |
pubmed-article:406901 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:406901 | pubmed:day | 1 | lld:pubmed |
pubmed-article:406901 | pubmed:volume | 163 | lld:pubmed |
pubmed-article:406901 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:406901 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:406901 | pubmed:pagination | 565-70 | lld:pubmed |
pubmed-article:406901 | pubmed:dateRevised | 2010-9-2 | lld:pubmed |
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pubmed-article:406901 | pubmed:year | 1977 | lld:pubmed |
pubmed-article:406901 | pubmed:articleTitle | Separation and properties of two arylamidases from rat cardiac-muscle extracts. | lld:pubmed |
pubmed-article:406901 | pubmed:publicationType | Journal Article | lld:pubmed |
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