| pubmed-article:3997861 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3997861 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:3997861 | lifeskim:mentions | umls-concept:C0028589 | lld:lifeskim |
| pubmed-article:3997861 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:3997861 | pubmed:dateCreated | 1985-7-9 | lld:pubmed |
| pubmed-article:3997861 | pubmed:abstractText | The in vivo cross-linking of proteins to DNA in intact Novikoff ascites hepatoma cells exposed to the chromium salt K2CrO4 was studied. DNA-protein complexes were assayed by high speed centrifugation of cells solubilized in buffered 4% sodium dodecyl sulfate and by electrophoretic identification of proteins associated with DNA-containing pellets. Further evidence of DNA-protein complexes, not dissociable in this buffer, was obtained by CsCl gradient centrifugation. Time dependence experiments showed that detectable cross-linking occurred after cells were exposed to chromium salt for at least 4 h, and the amount of DNA-protein complexes increased with longer incubation times. Complex formation occurred only with chromium salt concentrations of 200 microM or greater, and maximal cross-linking was effected at 5 mM. Immunotransfer methodology employing antibodies to nuclear matrix fraction and lamins was used to identify some of the polypeptides comprising the cross-linked complexes. These studies indicated specificity of chromium-induced complex formation within the nuclear protein fractions assayed. Our results document the ability of chromate to produce specific DNA-protein cross-links in living cells. | lld:pubmed |
| pubmed-article:3997861 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3997861 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3997861 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3997861 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3997861 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3997861 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3997861 | pubmed:author | pubmed-author:HnilicaL SLS | lld:pubmed |
| pubmed-article:3997861 | pubmed:author | pubmed-author:SchmidtW NWN | lld:pubmed |
| pubmed-article:3997861 | pubmed:author | pubmed-author:WedrychowskiA... | lld:pubmed |
| pubmed-article:3997861 | pubmed:author | pubmed-author:WattW EWE | lld:pubmed |
| pubmed-article:3997861 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3997861 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:3997861 | pubmed:volume | 260 | lld:pubmed |
| pubmed-article:3997861 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3997861 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3997861 | pubmed:pagination | 7150-5 | lld:pubmed |
| pubmed-article:3997861 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
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| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
| pubmed-article:3997861 | pubmed:meshHeading | pubmed-meshheading:3997861-... | lld:pubmed |
| pubmed-article:3997861 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3997861 | pubmed:articleTitle | Chromium-induced cross-linking of nuclear proteins and DNA. | lld:pubmed |
| pubmed-article:3997861 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3997861 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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