| pubmed-article:3926896 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0029341 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0003261 | lld:lifeskim |
| pubmed-article:3926896 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:3926896 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3926896 | pubmed:dateCreated | 1985-9-19 | lld:pubmed |
| pubmed-article:3926896 | pubmed:abstractText | The in vitro T cell-dependent antibody response of human lymphocytes to influenza virus X31 was used to study the role of T cell-derived lymphokines in antigen-specific responses. Supernatant from cultures of phytohaemagglutinin-stimulated, pooled human tonsil cells (PHA-MLR) was capable of replacing T cells and inducing T-depleted tonsil cells to secrete influenza-specific antibody. The T cell-replacing activity of PHA-MLR supernatant co-purified with interleukin 2 (IL 2) on Ultrogel AcA54 gel filtration and reversed phase-high performance liquid chromatography. PHA-MLR supernatant and IL 2 also enhanced B cell proliferation induced by anti-mu or Staphylococcal aureus strain Cowan I (SAC). A murine monoclonal antibody directed against the human IL 2 receptor (Mab 2A3) was used to completely block the enhancement of influenza-specific antibody production mediated by PHA-MLR supernatant, purified IL 2, and recombinant human IL 2. Mab 2A3 did not affect the T-independent B cell proliferation induced by anti-mu or SAC, but abrogated the enhancing effect of the PHA-MLR supernatant and IL 2 in this culture system. Immunofluorescence studies failed to demonstrate binding of Mab 2A3 to B cells activated by the X31 influenza virus and IL 2, or by SAC. By using Mab 2A3 to mask out IL 2 effects in the influenza-specific culture system, no other B cell differentiating activities were revealed in supernatants from lymphocytic cultures stimulated with a variety of mitogens. Thus, our results indicate that the production of influenza-specific antibodies by T-depleted human lymphocyte cultures is absolutely dependent on the presence of both antigen and IL 2. | lld:pubmed |
| pubmed-article:3926896 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3926896 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:3926896 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3926896 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3926896 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3926896 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:BoothR JRJ | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:PrestidgeR... | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:GillisSS | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:TanP LPL | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:DowerS KSK | lld:pubmed |
| pubmed-article:3926896 | pubmed:author | pubmed-author:WatsonJ DJD | lld:pubmed |
| pubmed-article:3926896 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3926896 | pubmed:volume | 135 | lld:pubmed |
| pubmed-article:3926896 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3926896 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3926896 | pubmed:pagination | 2128-33 | lld:pubmed |
| pubmed-article:3926896 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:meshHeading | pubmed-meshheading:3926896-... | lld:pubmed |
| pubmed-article:3926896 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3926896 | pubmed:articleTitle | Induction of antibody responses to influenza virus in human lymphocyte cultures. I. Role of interleukin 2. | lld:pubmed |
| pubmed-article:3926896 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3926896 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3926896 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3926896 | lld:pubmed |
