| pubmed-article:3894499 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3894499 | lifeskim:mentions | umls-concept:C0087111 | lld:lifeskim |
| pubmed-article:3894499 | lifeskim:mentions | umls-concept:C0021699 | lld:lifeskim |
| pubmed-article:3894499 | lifeskim:mentions | umls-concept:C0011740 | lld:lifeskim |
| pubmed-article:3894499 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
| pubmed-article:3894499 | lifeskim:mentions | umls-concept:C0079603 | lld:lifeskim |
| pubmed-article:3894499 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:3894499 | pubmed:dateCreated | 1985-9-4 | lld:pubmed |
| pubmed-article:3894499 | pubmed:abstractText | Immunofluorescence microscopy of cultured animal cells is often performed after detergent permeabilization of formaldehyde-fixed cellular membranes so that antibodies may have access to intracellular antigens. A comparison was made of the ability of several detergents, after formaldehyde fixation, to affect localization of intracellular proteins or to permeabilize different organelles to antibodies. Saponin, a detergent-like molecule that can permeabilize cholesterol-containing membranes, was also used. Four monoclonal antibodies were found to have a bright, discrete fluorescence localization with saponin alone, but were almost undetectable when the cells were treated with nonionic detergents such as Triton X-100 or NP-40. These immunoglobulin G antibodies included two against lysosomal membrane glycoproteins, one against an integral membrane protein found in the plasma membrane and endocytic vesicles, and one against a membrane protein in the endoplasmic reticulum and the nuclear envelope. However, antigens localized in mitochondria and the nucleus required the use of a detergent such as Triton X-100 for their detection. The detection of a number of other membrane or cytoplasmic proteins was unaffected by Triton X-100 treatment. It was concluded that nonionic detergents such as Triton X-100 cause artifactual loss of detection of some membrane proteins, and saponin is a favorable alternative reagent for immunofluorescence detection of intracellular membrane antigens in many organelles. | lld:pubmed |
| pubmed-article:3894499 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3894499 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3894499 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3894499 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3894499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3894499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3894499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3894499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3894499 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3894499 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3894499 | pubmed:issn | 0022-1554 | lld:pubmed |
| pubmed-article:3894499 | pubmed:author | pubmed-author:AugustJ TJT | lld:pubmed |
| pubmed-article:3894499 | pubmed:author | pubmed-author:WillinghamM... | lld:pubmed |
| pubmed-article:3894499 | pubmed:author | pubmed-author:HedmanKK | lld:pubmed |
| pubmed-article:3894499 | pubmed:author | pubmed-author:ChenJ WJW | lld:pubmed |
| pubmed-article:3894499 | pubmed:author | pubmed-author:GoldenthalK... | lld:pubmed |
| pubmed-article:3894499 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3894499 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:3894499 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3894499 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3894499 | pubmed:pagination | 813-20 | lld:pubmed |
| pubmed-article:3894499 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3894499 | pubmed:meshHeading | pubmed-meshheading:3894499-... | lld:pubmed |
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| pubmed-article:3894499 | pubmed:meshHeading | pubmed-meshheading:3894499-... | lld:pubmed |
| pubmed-article:3894499 | pubmed:meshHeading | pubmed-meshheading:3894499-... | lld:pubmed |
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| pubmed-article:3894499 | pubmed:meshHeading | pubmed-meshheading:3894499-... | lld:pubmed |
| pubmed-article:3894499 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3894499 | pubmed:articleTitle | Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins. | lld:pubmed |
| pubmed-article:3894499 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3894499 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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