| pubmed-article:3888956 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0314883 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0023689 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0332255 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0018164 | lld:lifeskim |
| pubmed-article:3888956 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:3888956 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3888956 | pubmed:dateCreated | 1985-7-19 | lld:pubmed |
| pubmed-article:3888956 | pubmed:abstractText | Partially digested chromosomal DNA of Bacillus brevis ATCC 9999, a producer of the cyclic peptide antibiotic gramicidin S, was ligated into the BamHI site of the Escherichia coli expression vector pUR2-Bam. The ligated molecules were used to transfer E. coli to ampicillin resistance. Of 5 X 10(3) colonies tested by in situ immunoassay for a cross-reaction with antibodies against the gramicidin S synthetase 2, 6 colonies were found to be immunoreactive. A clone designated MK2, which had a 3.9-kilobase insert of B. brevis DNA, directed in E. coli under the lac promoter control the synthesis of polypeptides that were cross-reactive with the antibody to the gramicidin S synthetase 2. Partial purification of the gene products by gel filtration revealed a major fraction with an approximate molecular weight of 140,000 and with specific ornithine-dependent ATP-32PPi and 2'-dATP-32PPi exchange activities. These unique activities of the gramicidin S synthetase 2 were not detected in the E. coli strain harboring the vector. | lld:pubmed |
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| pubmed-article:3888956 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3888956 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3888956 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3888956 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3888956 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3888956 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:3888956 | pubmed:author | pubmed-author:KleinkaufHH | lld:pubmed |
| pubmed-article:3888956 | pubmed:author | pubmed-author:KrauseMM | lld:pubmed |
| pubmed-article:3888956 | pubmed:author | pubmed-author:MarahielM AMA | lld:pubmed |
| pubmed-article:3888956 | pubmed:author | pubmed-author:von DöhrenHH | lld:pubmed |
| pubmed-article:3888956 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3888956 | pubmed:volume | 162 | lld:pubmed |
| pubmed-article:3888956 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3888956 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3888956 | pubmed:pagination | 1120-5 | lld:pubmed |
| pubmed-article:3888956 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3888956 | pubmed:meshHeading | pubmed-meshheading:3888956-... | lld:pubmed |
| pubmed-article:3888956 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3888956 | pubmed:articleTitle | Molecular cloning of an ornithine-activating fragment of the gramicidin S synthetase 2 gene from Bacillus brevis and its expression in Escherichia coli. | lld:pubmed |
| pubmed-article:3888956 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3888956 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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