| pubmed-article:3872884 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C0812443 | lld:lifeskim |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:3872884 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:3872884 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:3872884 | pubmed:dateCreated | 1985-5-28 | lld:pubmed |
| pubmed-article:3872884 | pubmed:abstractText | Human plasma obtained from patients with hypomegakaryocytic thrombocytopenia contains a factor that promotes megakaryocyte colony formation by normal human marrow cells. This megakaryocyte colony-stimulating factor was purified from such a plasma specimen. A four-step purification scheme which included ammonium sulfate precipitation, diethylaminoethyl-Sepharose chromatography, affinity chromatography on wheat germ lectin-Sepharose 6MB, and reverse-phase high performance liquid chromatography resulted in a recovery of 16.6% of the initial biological activity and an increase in specific activity by 3,489-fold. The purified protein produced a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified megakaryocyte colony-stimulating factor was capable of promoting megakaryocyte colony formation at a concentration of 7.6 X 10(-8) M. Megakaryocyte colony-stimulating factor was shown to be a glycoprotein and had an apparent 46,000 mol wt. Deglycosylation of megakaryocyte colony-stimulating factor by treatment with trifluoromethane-sulfonate resulted in the loss of its ability to promote megakaryocyte colony formation. Megakaryocyte colony-stimulating factor appears to be an important regulator of in vitro human megakaryocytopoiesis at the level of the colony-forming unit megakaryocyte and may be of importance physiologically. | lld:pubmed |
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| pubmed-article:3872884 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3872884 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:3872884 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3872884 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3872884 | pubmed:issn | 0021-9738 | lld:pubmed |
| pubmed-article:3872884 | pubmed:author | pubmed-author:YangH HHH | lld:pubmed |
| pubmed-article:3872884 | pubmed:author | pubmed-author:BrunoEE | lld:pubmed |
| pubmed-article:3872884 | pubmed:author | pubmed-author:HoffmanRR | lld:pubmed |
| pubmed-article:3872884 | pubmed:author | pubmed-author:StranevaJ EJE | lld:pubmed |
| pubmed-article:3872884 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3872884 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:3872884 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3872884 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3872884 | pubmed:pagination | 1174-82 | lld:pubmed |
| pubmed-article:3872884 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3872884 | pubmed:meshHeading | pubmed-meshheading:3872884-... | lld:pubmed |
| pubmed-article:3872884 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3872884 | pubmed:articleTitle | Purification and partial characterization of a megakaryocyte colony-stimulating factor from human plasma. | lld:pubmed |
| pubmed-article:3872884 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3872884 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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