pubmed-article:3843705 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:3843705 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
pubmed-article:3843705 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
pubmed-article:3843705 | lifeskim:mentions | umls-concept:C0053554 | lld:lifeskim |
pubmed-article:3843705 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:3843705 | pubmed:dateCreated | 1986-2-7 | lld:pubmed |
pubmed-article:3843705 | pubmed:abstractText | Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in the reaction of protein with alkaline Cu2+ (biuret reaction). The color produced from this reaction is stable and increases in a proportional fashion over a broad range of increasing protein concentrations. When compared to the method of Lowry et al., the results reported here demonstrate a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts. The stability of the reagent and resulting chromophore also allows for a simplified, one-step analysis and an enhanced flexibility in protocol selection. This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique. | lld:pubmed |
pubmed-article:3843705 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3843705 | pubmed:language | eng | lld:pubmed |
pubmed-article:3843705 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3843705 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:3843705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3843705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3843705 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3843705 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:3843705 | pubmed:month | Oct | lld:pubmed |
pubmed-article:3843705 | pubmed:issn | 0003-2697 | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:SmithP KPK | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:OlsonB JBJ | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:MalliaA KAK | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:FujimotoE KEK | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:HermansonG... | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:KlenkD CDC | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:KrohnR IRI | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:GartnerF HFH | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:ProvenzanoM... | lld:pubmed |
pubmed-article:3843705 | pubmed:author | pubmed-author:GoekeN MNM | lld:pubmed |
pubmed-article:3843705 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:3843705 | pubmed:volume | 150 | lld:pubmed |
pubmed-article:3843705 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:3843705 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:3843705 | pubmed:pagination | 76-85 | lld:pubmed |
pubmed-article:3843705 | pubmed:dateRevised | 2000-12-18 | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:meshHeading | pubmed-meshheading:3843705-... | lld:pubmed |
pubmed-article:3843705 | pubmed:year | 1985 | lld:pubmed |
pubmed-article:3843705 | pubmed:articleTitle | Measurement of protein using bicinchoninic acid. | lld:pubmed |
pubmed-article:3843705 | pubmed:publicationType | Journal Article | lld:pubmed |
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