| pubmed-article:3839315 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C0040291 | lld:lifeskim |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C0027893 | lld:lifeskim |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C0597879 | lld:lifeskim |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C0034580 | lld:lifeskim |
| pubmed-article:3839315 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:3839315 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:3839315 | pubmed:dateCreated | 1985-8-16 | lld:pubmed |
| pubmed-article:3839315 | pubmed:abstractText | A sensitive and specific radioimmunoassay was developed to determine the occurrence and concentration of neuropeptide Y (NPY) in plasma and tissue extracts. Furthermore, NPY-like immunoreactivity (NPY-Li) was characterized by means of three different chromatographic systems. The NPY antiserum used (NI) did not cross-react with related peptides of the pancreatic polypeptide family except avian pancreatic polypeptide (1% cross-reactivity). Unextracted plasma contained high molecular weight proteins which interfered in the assay. Acid ethanol extraction removed this protein interference allowing a 90% recovery of NPY-Li. The content of NPY-Li in human plasma from healthy subjects was close to or below the detection limit (less than 22 pmol/l). Sympathetic nerve stimulation in the cat increased the output of NPY-Li from the splenic vein suggesting the release of this peptide upon sympathetic activation. The major peak of NPY-Li in spleen extracts and splenic vein plasma co-eluted with synthetic porcine NPY and a minor peak with larger Stokes radius was also present. The present radioimmunoassay enables further studies on the physiological and pathophysiological role of NPY. | lld:pubmed |
| pubmed-article:3839315 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3839315 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3839315 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3839315 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3839315 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3839315 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3839315 | pubmed:issn | 0036-5513 | lld:pubmed |
| pubmed-article:3839315 | pubmed:author | pubmed-author:LundbergJ MJM | lld:pubmed |
| pubmed-article:3839315 | pubmed:author | pubmed-author:HemsénAA | lld:pubmed |
| pubmed-article:3839315 | pubmed:author | pubmed-author:Theodorsson-N... | lld:pubmed |
| pubmed-article:3839315 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3839315 | pubmed:volume | 45 | lld:pubmed |
| pubmed-article:3839315 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3839315 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3839315 | pubmed:pagination | 355-65 | lld:pubmed |
| pubmed-article:3839315 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:3839315 | pubmed:meshHeading | pubmed-meshheading:3839315-... | lld:pubmed |
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| pubmed-article:3839315 | pubmed:meshHeading | pubmed-meshheading:3839315-... | lld:pubmed |
| pubmed-article:3839315 | pubmed:meshHeading | pubmed-meshheading:3839315-... | lld:pubmed |
| pubmed-article:3839315 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3839315 | pubmed:articleTitle | Radioimmunoassay for neuropeptide Y (NPY): chromatographic characterization of immunoreactivity in plasma and tissue extracts. | lld:pubmed |
| pubmed-article:3839315 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3839315 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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