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pubmed-article:3817155pubmed:abstractTextImmunoperoxidase electrophoresis was applied to the plasma of a patient showing a high level of prolactin (PRL) secreted by a pituitary adenoma. Two PRL monomers were detected with an anti-hPRL antiserum: a major 22 kDa form and a minor 25 kDa form. Concanavalin A-Sepharose 4B chromatography revealed that the 25 kDa form was a glycosylated variant of PRL. Incubation of this variant with endoglycosidase F led to its transformation into the 22 kDa form.lld:pubmed
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pubmed-article:3817155pubmed:articleTitleDetection and enzymatic deglycosylation of a glycosylated variant of prolactin in human plasma.lld:pubmed
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