| pubmed-article:381107 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0016937 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:381107 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:381107 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:381107 | pubmed:dateCreated | 1979-10-26 | lld:pubmed |
| pubmed-article:381107 | pubmed:abstractText | This report describes the construction and isolation of a plasmid, derived from pBR322, which carries a BglII restriction fragment of DNA containing the galactokinase gene from Saccharomyces cerevisiae. This was accomplished by the following procedure: (1) Purified galactokinase mRNA, labelled with 125I, was hybridized to BglII digests of yeast DNA employing Southern's filter transfer technique to identify a restriction fragment containing the galactokinase gene. (2) This fragment was partially purified by agarose gel electrophoresis, ligated into the BamHI site of pBR322 and transformed into Escherichia coli to generate a clone bank containing the galactokinase gene. (3) This bank was screened by in situ colony hybridization with galactokinase mRNA resulting in the identification of a plasmid carrying this gene. This plasmid DNA hybridized with the galactokinase mRNA to the same extent in the presence of absence of a large excess of unlabelled mRNA from cells that were not induced for galactokinase synthesis, while the same amount of unlabelled galactose-induced mRNA reduced the hybridization by 95%. When this plasmid was introduced into an E. coli strain deleted for the galactose operon it caused the synthesis of low levels of yeast galactokinase activity. | lld:pubmed |
| pubmed-article:381107 | pubmed:language | eng | lld:pubmed |
| pubmed-article:381107 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:381107 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:381107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:381107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:381107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:381107 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:381107 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:381107 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:381107 | pubmed:author | pubmed-author:WilsonD BDB | lld:pubmed |
| pubmed-article:381107 | pubmed:author | pubmed-author:SchellM AMA | lld:pubmed |
| pubmed-article:381107 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:381107 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:381107 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:381107 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:381107 | pubmed:pagination | 291-303 | lld:pubmed |
| pubmed-article:381107 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-S... | lld:pubmed |
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| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-T... | lld:pubmed |
| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-N... | lld:pubmed |
| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-P... | lld:pubmed |
| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-D... | lld:pubmed |
| pubmed-article:381107 | pubmed:meshHeading | pubmed-meshheading:381107-G... | lld:pubmed |
| pubmed-article:381107 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:381107 | pubmed:articleTitle | Cloning and expression of the yeast galactokinase gene in an Escherichia coli plasmid. | lld:pubmed |
| pubmed-article:381107 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:381107 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:381107 | lld:pubmed |
