| pubmed-article:3793188 | pubmed:abstractText | Mutagens present in crude samples such as heated glucose can be detected or identified by means of the adducts formed upon reaction with a fluorescent guanosine derivative (FG) or isopropylideneguanosine (IPG). After the reaction of IPG with heated glucose, two adducts were isolated by high-performance liquid chromatography. One of the adducts was identified as the cyclic adduct formed between IPG and glyoxal. Mesoxaldialdehyde, which is structurally related to glyoxal, also produced a cyclic IPG-adduct and showed mutagenic activity in Salmonella typhimurium strain TA100. The other adduct isolated from the reaction mixture of IPG and heated glucose was 8-hydroxy-IPG. Various reagents which generate oxygen radicals were effective in the hydroxylation of guanosine derivatives at the C-8 position. These reagents also cause hydroxylation of guanine residues in DNA. | lld:pubmed |
