| pubmed-article:3755432 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0521449 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0025251 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:3755432 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:3755432 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:3755432 | pubmed:dateCreated | 1986-9-17 | lld:pubmed |
| pubmed-article:3755432 | pubmed:abstractText | The activation of human neutrophils by chemotactic peptides evokes a rapid change in membrane potential and an increase in cytoplasmic Ca2+ levels. These events are followed up to a minute later by detectable levels of microbicidal agents formed by the oxidative burst. Except for the latter, the sequence of events has remained unclear. We report here that a new fluorescent Ca2+ indicator developed by R. Tsien, Indo-1, has allowed us to resolve the temporal relationship between the rapid and transient cytoplasmic Ca2+ rise and the membrane potential change and to do so on very small samples by using a fluorescence-activated cell sorter. We have adapted a FACS 440 for simultaneous single cell membrane depolarization and cytoplasmic [Ca2+] detection in human neutrophils upon stimulation with formyl-methionyl-leucyl-phenylalanine (fMLP). A membrane potential probe, dipentyloxacarbocyanine, allows us to determine that the membrane potential change is fMLP dose-dependent and apparently biphasic. The depolarization is maximal 40 s after stimulation. In contrast, cytosolic [Ca2+], while fMLP-dose dependent, is maximal at 10 s and already decreasing rapidly when the cell has reached its lowest potential. It can be measured with Indo-1 which has a fluorescence emission (lambda ex = 357 nm) maximum at 485 nm when Ca2+-free and 405 nm when Ca2+-liganded. The ratio of these fluorescences may then be calibrated in terms of cytoplasmic Ca2+ levels. Thus, Ca2+ release into the cytoplasm becomes the earliest evidence of neutrophil stimulation by fMLP and occurs in close association with an apparent membrane hyperpolarization. | lld:pubmed |
| pubmed-article:3755432 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3755432 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3755432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3755432 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3755432 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3755432 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3755432 | pubmed:author | pubmed-author:SimonsE RER | lld:pubmed |
| pubmed-article:3755432 | pubmed:author | pubmed-author:LazzariK GKG | lld:pubmed |
| pubmed-article:3755432 | pubmed:author | pubmed-author:ProtoP JPJ | lld:pubmed |
| pubmed-article:3755432 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3755432 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:3755432 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:3755432 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3755432 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3755432 | pubmed:pagination | 9710-3 | lld:pubmed |
| pubmed-article:3755432 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:meshHeading | pubmed-meshheading:3755432-... | lld:pubmed |
| pubmed-article:3755432 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3755432 | pubmed:articleTitle | Simultaneous measurement of stimulus-induced changes in cytoplasmic Ca2+ and in membrane potential of human neutrophils. | lld:pubmed |
| pubmed-article:3755432 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3755432 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3755432 | lld:pubmed |
