Linked Life Data

3747823

Source:http://linkedlifedata.com/resource/pubmed/id/3747823

Statements in which the resource exists.
SubjectPredicateObjectContext
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pubmed-article:3747823pubmed:abstractText[guanidino-13C]Creatine has been synthesized by reaction involving synthesis of [13C]cyanamide followed by its condensation with sarcosine. The creatine guanidino carbon 13C NMR frequency shifts upon the formation of phosphocreatine by 0.83 ppm with a splitting (2JCP = 3.3 Hz) due to two-bond phosphorus-carbon coupling. The high 13C enrichment (99%) at the guanidino carbon of creatine gave rise to an adequate signal-to-noise ratio for both creatine and phosphocreatine so that a study of the time course of the creatine phosphokinase reaction could be performed and provided the means for a determination of the Km (15.5 mM) of the reaction.lld:pubmed
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pubmed-article:3747823pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3747823pubmed:articleTitleSynthesis of [guanidino-13C]creatine and measurement of the creatine phosphokinase reaction in vitro by 13C NMR spectroscopy.lld:pubmed
pubmed-article:3747823pubmed:publicationTypeJournal Articlelld:pubmed
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