| pubmed-article:3734459 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0016055 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:3734459 | lifeskim:mentions | umls-concept:C1516240 | lld:lifeskim |
| pubmed-article:3734459 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3734459 | pubmed:dateCreated | 1986-9-17 | lld:pubmed |
| pubmed-article:3734459 | pubmed:abstractText | A new solid-phase enzyme immunoassay measuring the gelatin-binding capacity of plasma fibronectin has been developed. This assay is based on the direct and high-affinity interaction between fibronectin and gelatin coated to polyvinyl chloride plates. The amount of fibronectin bound to gelatin is then measured by sequential incubation with a specific rabbit anti-human fibronectin antiserum, with horseradish peroxidase-conjugated goat anti-rabbit IgG antibodies and with substrate. The final degradation of the substrate is read at 492-650 nm in an ELISA processor. The assay allows the accurate detection of fibronectin concentrations ranging from 1 to 20 micrograms/ml, is inhibited by the addition of gelatin to plasma, is highly reproducible (interplate CV less than 10%), requires 100 microliter of plasma only and has been fully automated. Significant linear correlations were noted between total antigenic fibronectin (measured by laser nephelometry) and fibronectin gelatin-binding capacity in plasma from 310 blood donors. Both parameters were higher in men than in women and significantly increased according to age. Dissociation between immunoreactive fibronectin and fibronectin gelatin-binding capacity was observed in two polytraumatized patients. This enzyme immunoassay therefore provides a new method to investigate functional alterations of the gelatin-binding domain of fibronectin in various pathological conditions. | lld:pubmed |
| pubmed-article:3734459 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3734459 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3734459 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3734459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3734459 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3734459 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3734459 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3734459 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:LangBB | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:AdamAA | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:ClossetJJ | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:MahieuPP | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:FoidartJ MJM | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:CalayGG | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:FoidartJJ | lld:pubmed |
| pubmed-article:3734459 | pubmed:author | pubmed-author:DamasPP | lld:pubmed |
| pubmed-article:3734459 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3734459 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:3734459 | pubmed:volume | 91 | lld:pubmed |
| pubmed-article:3734459 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3734459 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3734459 | pubmed:pagination | 205-11 | lld:pubmed |
| pubmed-article:3734459 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:meshHeading | pubmed-meshheading:3734459-... | lld:pubmed |
| pubmed-article:3734459 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3734459 | pubmed:articleTitle | An enzyme-linked immunoassay for direct measurement of the gelatin-binding capacity of human plasma fibronectin. | lld:pubmed |
| pubmed-article:3734459 | pubmed:publicationType | Journal Article | lld:pubmed |
