| pubmed-article:3718997 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0015684 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:3718997 | lifeskim:mentions | umls-concept:C1306918 | lld:lifeskim |
| pubmed-article:3718997 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3718997 | pubmed:dateCreated | 1986-7-28 | lld:pubmed |
| pubmed-article:3718997 | pubmed:abstractText | Uptake of long-chain fatty acids by short-term cultured hepatocytes was studied. Rat hepatocytes, which were cultured for 16 h on plastic dishes (3.6 X 10(6) cells/dish), were incubated with [3H]oleate in the presence of various concentrations of bovine serum albumin as a function of the concentration of unbound [3H]oleate in the medium. At 37 degrees C initial uptake velocity (V0) was saturable (Km = 9 X 10(-8) M; Vmax = 835 pmol/min per mg protein). V0 was temperature dependent with an optimum at 37 degrees C and markedly reduced at 4 degrees C and 70 degrees C. To evaluate the biologic significance of a previously isolated rat liver plasma membrane fatty acid-binding protein as putative carrier protein in the hepatocellular uptake of fatty acids, cultured hepatocytes were treated with a monospecific rabbit antibody (IgG-fraction) to this membrane protein or the IgG-fraction of the pre-immune serum as controls. Uptake kinetics of [3H]oleate in antibody pretreated short-term cultured hepatocytes revealed a depression of Vmax by 70%, while Km was only reduced by 16% compared to controls, indicating a predominant non-competitive type of inhibition. V0 of a variety of long-chain fatty acids (oleic acid, arachidonic acid, palmitic acid, stearic acid) was reduced by 56-69%, while V0 of [35S]sulfobromophthalein, [3H]cholic acid and [14C]taurocholic acid remained unaltered. These data support the concept that in the system of cultured hepatocytes, uptake of long-chain fatty acids is mediated by the rat liver plasma membrane fatty acid-binding protein. | lld:pubmed |
| pubmed-article:3718997 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3718997 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3718997 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3718997 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3718997 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3718997 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:3718997 | pubmed:author | pubmed-author:StremmelWW | lld:pubmed |
| pubmed-article:3718997 | pubmed:author | pubmed-author:TheilmannLL | lld:pubmed |
| pubmed-article:3718997 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3718997 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:3718997 | pubmed:volume | 877 | lld:pubmed |
| pubmed-article:3718997 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3718997 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3718997 | pubmed:pagination | 191-7 | lld:pubmed |
| pubmed-article:3718997 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:3718997 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3718997 | pubmed:articleTitle | Selective inhibition of long-chain fatty acid uptake in short-term cultured rat hepatocytes by an antibody to the rat liver plasma membrane fatty acid-binding protein. | lld:pubmed |
| pubmed-article:3718997 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3718997 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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