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pubmed-article:3706738pubmed:abstractTextBoric acid has been known to make a complex with 2-hydroxycarboxylic acids. Based on this principle, we have developed a new method for isolation of vanillylmandelic acid, p-hydroxymandelic acid, vanillyllactic acid, and p-hydroxyphenyllactic acid in urine. The technique involves two steps: extraction of lipophilic compounds from urine with n-butanol and selective isolation of 2-hydroxy acids in the n-butanol phase on a phenylboronate gel column. 2-Hydroxy acids were eluted from the column with 1.5 ml of 2% acetyl chloride in methanol with the recoveries of ca. 70%. According to analysis of urinary extract by gas chromatography--mass spectrometry, the mean excretion rates of the above four compounds in 12 healthy subjects were 3.68, 1.93, 0.091, and 1.08 mg/day, respectively.lld:pubmed
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pubmed-article:3706738pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3706738pubmed:year1986lld:pubmed
pubmed-article:3706738pubmed:articleTitleIsolation of 2-hydroxycarboxylic acids with a boronate affinity gel.lld:pubmed
pubmed-article:3706738pubmed:publicationTypeJournal Articlelld:pubmed