pubmed-article:3667757 | pubmed:abstractText | The preparative separation of saikosaponins a, c, and d from Bupleurum falcatum was performed by high-performance liquid chromatography on a 100 X 11 cm I.D. axially compressed column, packed with 20-micron ODS silica gel using a step gradient with mixtures of acetonitrile and water as the mobile phase. Gram quantities of pure compounds were obtained in a single run. | lld:pubmed |