| pubmed-article:3624877 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3624877 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:3624877 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:3624877 | lifeskim:mentions | umls-concept:C1160466 | lld:lifeskim |
| pubmed-article:3624877 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3624877 | pubmed:dateCreated | 1987-10-5 | lld:pubmed |
| pubmed-article:3624877 | pubmed:abstractText | DNA was conjugated to sheep red blood cells (SRBC) by chemical methods using CrCl3, poly-L-lysine or methylated bovine serum albumin as conjugation agents and by a physical method where conjugation was accomplished by incubation at 45 degrees C. The degree of conjugation was estimated using 32P-DNA (mean size 1 kbase pairs). Employing the CrCl3 method 5.8 +/- 3.6 micrograms DNA were conjugated per 10(8) SRBC at a concentration of 70 micrograms DNA/10(8) cells. At the same DNA concentration in the incubation medium 3.0 +/- 0.6 microgram DNA/10(8) cells were conjugated by poly-L-lysine, 4.1 +/- 0.8 microgram DNA/10(8) cells by methylated bovine serum albumin and approximately 4 micrograms DNA/10(8) cells when the cells were incubated at 45 degrees C. Cells conjugated with DNA by CrCl3 showed linearly increasing conjugation with increasing concentration of DNA. Cells conjugated by poly-L-lysine (pLL) or methylated bovine serum albumin seemed to be saturated by DNA at 30 micrograms DNA per 10(8) cells. At 45 degrees C the spontaneous adhesion of DNA to SRBC increased in the concentration range investigated. The degree of conjugation of DNA to SRBC was influenced by pH, and Ca2+.pLL-conjugated DNA-SRBC, but none of the other preparations were lysed in a hemolytic assay using anti-DNA antiserum from a patient with systemic lupus erythematosus. | lld:pubmed |
| pubmed-article:3624877 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3624877 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3624877 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3624877 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3624877 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:3624877 | pubmed:author | pubmed-author:AndersenVV | lld:pubmed |
| pubmed-article:3624877 | pubmed:author | pubmed-author:KochCC | lld:pubmed |
| pubmed-article:3624877 | pubmed:author | pubmed-author:Hellung-Larse... | lld:pubmed |
| pubmed-article:3624877 | pubmed:author | pubmed-author:LoftagerM KMK | lld:pubmed |
| pubmed-article:3624877 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3624877 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:3624877 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:3624877 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3624877 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3624877 | pubmed:pagination | 65-9 | lld:pubmed |
| pubmed-article:3624877 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:meshHeading | pubmed-meshheading:3624877-... | lld:pubmed |
| pubmed-article:3624877 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3624877 | pubmed:articleTitle | Conjugation of DNA to erythrocytes. | lld:pubmed |
| pubmed-article:3624877 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3624877 | lld:pubmed |
