| pubmed-article:3609183 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C1947989 | lld:lifeskim |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:3609183 | lifeskim:mentions | umls-concept:C1627358 | lld:lifeskim |
| pubmed-article:3609183 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:3609183 | pubmed:dateCreated | 1987-9-3 | lld:pubmed |
| pubmed-article:3609183 | pubmed:abstractText | Recombinant human insulin-like growth factor I (IGF-I) increased human and murine erythropoietic colony formation in serum-free culture. In order to investigate the effects of purified factors such as IGF-I on hemopoietic progenitor cells, we have established a serum-free culture system which supports the clonal growth of CFU-E- and BFU-E-derived colonies. Exogenously supplied ingredients were bovine serum albumin (BSA), transferrin, lipid suspensions, 2-mercaptoethanol, and recombinant human erythropoietin (epo). Among these, BSA and cholesterol were found to be essential ingredients. The optimum concentration of BSA sufficient to grow BFU-E was 3%. Erythroid colony and burst formation of human and murine marrow cells was enhanced twofold (p less than 0.05) by a physiological concentration of recombinant human IGF-I. Potentiation was observed in a dose-dependent manner between 10(-9) and 10(-7) M. A few murine CFU-E colonies were formed in the absence of epo. These results suggest that IGF-I has a supportive effect on the proliferation and differentiation of erythroid precursor cells stimulated by epo and that its action is synergistic with that of epo. | lld:pubmed |
| pubmed-article:3609183 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3609183 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3609183 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3609183 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3609183 | pubmed:issn | 0301-472X | lld:pubmed |
| pubmed-article:3609183 | pubmed:author | pubmed-author:TakakuFF | lld:pubmed |
| pubmed-article:3609183 | pubmed:author | pubmed-author:UrabeAA | lld:pubmed |
| pubmed-article:3609183 | pubmed:author | pubmed-author:RottN NNN | lld:pubmed |
| pubmed-article:3609183 | pubmed:author | pubmed-author:AkahaneKK | lld:pubmed |
| pubmed-article:3609183 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3609183 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:3609183 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3609183 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3609183 | pubmed:pagination | 797-802 | lld:pubmed |
| pubmed-article:3609183 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:3609183 | pubmed:meshHeading | pubmed-meshheading:3609183-... | lld:pubmed |
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| pubmed-article:3609183 | pubmed:meshHeading | pubmed-meshheading:3609183-... | lld:pubmed |
| pubmed-article:3609183 | pubmed:meshHeading | pubmed-meshheading:3609183-... | lld:pubmed |
| pubmed-article:3609183 | pubmed:meshHeading | pubmed-meshheading:3609183-... | lld:pubmed |
| pubmed-article:3609183 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3609183 | pubmed:articleTitle | Pure erythropoietic colony and burst formations in serum-free culture and their enhancement by insulin-like growth factor I. | lld:pubmed |
| pubmed-article:3609183 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3609183 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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