| pubmed-article:3606647 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C1749467 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0001128 | lld:lifeskim |
| pubmed-article:3606647 | lifeskim:mentions | umls-concept:C0006732 | lld:lifeskim |
| pubmed-article:3606647 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:3606647 | pubmed:dateCreated | 1987-8-19 | lld:pubmed |
| pubmed-article:3606647 | pubmed:abstractText | Sorcin (soluble resistance-related calcium-binding protein), an acidic (pI = 5.7) protein (Mr approximately 20 kDa) previously designated V19, was originally identified in cells selected for high levels of resistance to vincristine. Two-dimensional gel electrophoresis and/or Western blot techniques now show sorcin to be overproduced in cells selected for resistance to actinomycin D (QUA/ADj), colchicine (CHRC5), and adriamycin (BE(2)-C/ADR). Not all cell lines selected for resistance to these drugs overproduced sorcin; e.g. cells of an independently selected actinomycin D-resistant subline of QUA, QUA/ADsx, did not contain increased amounts of sorcin. Sorcin was purified by preparative gel electrophoresis from QUA/ADj cells and used to generate specific antiserum in chickens. By Western blot analyses the antiserum was shown to recognize sorcin in QUA/ADj and in vincristine-resistant mouse and Chinese hamster lung, colchicine-resistant Chinese hamster ovary, and adriamycin-resistant human neuroblastoma lines. Low level expression of the protein was detectable in control, drug-sensitive cells. Direct binding assays with 45Ca2+ showed that sorcin was a calcium-binding protein. QUA/ADj cells contained increased numbers of double minute chromosomes (DMs), cytogenetic indicators of gene amplification. As found for two other multidrug-resistant sublines, sorcin overproduction in QUA/ADj cells may be the result of amplification of the sorcin-encoding gene. The overproduction of this protein in multidrug-resistant cells of various species implies that sorcin plays a role in expression of the resistant phenotype. | lld:pubmed |
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| pubmed-article:3606647 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3606647 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3606647 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3606647 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3606647 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3606647 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:3606647 | pubmed:author | pubmed-author:MeyersM BMB | lld:pubmed |
| pubmed-article:3606647 | pubmed:author | pubmed-author:BiedlerJ LJL | lld:pubmed |
| pubmed-article:3606647 | pubmed:author | pubmed-author:SpenglerB ABA | lld:pubmed |
| pubmed-article:3606647 | pubmed:author | pubmed-author:ChangT DTD | lld:pubmed |
| pubmed-article:3606647 | pubmed:author | pubmed-author:SchneiderK... | lld:pubmed |
| pubmed-article:3606647 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3606647 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3606647 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:3606647 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3606647 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3606647 | pubmed:pagination | 2373-80 | lld:pubmed |
| pubmed-article:3606647 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3606647 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3606647 | pubmed:articleTitle | Sorcin (V19), a soluble acidic calcium-binding protein overproduced in multidrug-resistant cells. Identification of the protein by anti-sorcin antibody. | lld:pubmed |
| pubmed-article:3606647 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3606647 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3606647 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3606647 | lld:pubmed |
