| pubmed-article:3601675 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3601675 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:3601675 | lifeskim:mentions | umls-concept:C0028606 | lld:lifeskim |
| pubmed-article:3601675 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:3601675 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:3601675 | pubmed:dateCreated | 1987-8-20 | lld:pubmed |
| pubmed-article:3601675 | pubmed:abstractText | The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these experiments reveal that the hybridization efficiencies of support-bound oligonucleotides were 75-80% and 40-50% for single-stranded oligonucleotide targets and long double-stranded targets, respectively. These hybridization efficiencies are dependent upon prehybridizing the support-bound oligonucleotides with dextran sulfate. In addition, comparisons of the relative hybridization efficiencies of the support-bound oligonucleotide and nitrocellulose-based systems have been made which indicate a retention of 13-28% of target sequences on the filters and a detection efficiency of 8-20%. | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3601675 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3601675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3601675 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3601675 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3601675 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:3601675 | pubmed:author | pubmed-author:DavisG RGR | lld:pubmed |
| pubmed-article:3601675 | pubmed:author | pubmed-author:GingerasT RTR | lld:pubmed |
| pubmed-article:3601675 | pubmed:author | pubmed-author:KwohD YDY | lld:pubmed |
| pubmed-article:3601675 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3601675 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:3601675 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:3601675 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3601675 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3601675 | pubmed:pagination | 5373-90 | lld:pubmed |
| pubmed-article:3601675 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:meshHeading | pubmed-meshheading:3601675-... | lld:pubmed |
| pubmed-article:3601675 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3601675 | pubmed:articleTitle | Hybridization properties of immobilized nucleic acids. | lld:pubmed |
| pubmed-article:3601675 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3601675 | lld:pubmed |
