| pubmed-article:3584132 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3584132 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:3584132 | lifeskim:mentions | umls-concept:C0035298 | lld:lifeskim |
| pubmed-article:3584132 | lifeskim:mentions | umls-concept:C1419391 | lld:lifeskim |
| pubmed-article:3584132 | lifeskim:mentions | umls-concept:C0031739 | lld:lifeskim |
| pubmed-article:3584132 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:3584132 | pubmed:dateCreated | 1987-7-2 | lld:pubmed |
| pubmed-article:3584132 | pubmed:abstractText | 11-cis-Retinaldehyde bound to cellular retinaldehyde-binding protein (CRALBP) is unaffected in bovine eyecup preparations by illumination that bleaches approximately 70% of the rhodopsin. Illumination of retinal homogenates to which CRALBP X [3H]11-cis-retinaldehyde had been added did not result in a reduction of the specific activity of recovered 11-cis-retinaldehyde, ruling out a bleaching regeneration cycle. The quantum efficiency of photoisomerization for CRALBP X 11-cis-retinaldehyde was determined by comparing the rate of photoisomerization of 11-cis-retinaldehyde bound to purified CRALBP and opsin. The low value obtained (0.07), coupled with a low molar extinction coefficient (15,400 M-1 cm-1), results in a photosensitivity only about 4% that of rhodopsin. CRALBP binds 9-cis- and 11-cis-retinaldehyde, producing complexes with absorption maxima at 405 and 425 nm, respectively. No complexes were detected with 13-cis- and all-trans-retinaldehyde. Following incubation of CRALBP X 11-cis-retinol with an equimolar mixture of 9-, 11-, 13-cis-, and all-trans-retinaldehydes, only 11-cis-retinaldehyde and residual 11-cis-retinol are present on the protein following separation from excess retinoids. A similar result is obtained following incubation of CRALBP X 11-cis-retinol with mixtures of 9- and 11-cis-retinaldehyde ranging in composition from 9:1 to 1:9 (9-cis-:11-cis-,mol/mol). The results indicate that CRALBP X 11-cis-retinol is sufficiently stereoselective in its binding properties to warrant consideration as a component of the mechanism for the generation of 11-cis-retinaldehyde in the dark. | lld:pubmed |
| pubmed-article:3584132 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3584132 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3584132 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3584132 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3584132 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3584132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3584132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3584132 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3584132 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:3584132 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3584132 | pubmed:author | pubmed-author:SaariJ CJC | lld:pubmed |
| pubmed-article:3584132 | pubmed:author | pubmed-author:BredbergD LDL | lld:pubmed |
| pubmed-article:3584132 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3584132 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:3584132 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:3584132 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3584132 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3584132 | pubmed:pagination | 7618-22 | lld:pubmed |
| pubmed-article:3584132 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
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| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:meshHeading | pubmed-meshheading:3584132-... | lld:pubmed |
| pubmed-article:3584132 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3584132 | pubmed:articleTitle | Photochemistry and stereoselectivity of cellular retinaldehyde-binding protein from bovine retina. | lld:pubmed |
| pubmed-article:3584132 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3584132 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3584132 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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