| pubmed-article:3562241 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C1257890 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0020944 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0001992 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0007066 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:3562241 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:3562241 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:3562241 | pubmed:dateCreated | 1987-5-21 | lld:pubmed |
| pubmed-article:3562241 | pubmed:abstractText | A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter. | lld:pubmed |
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| pubmed-article:3562241 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3562241 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3562241 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3562241 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3562241 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3562241 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:DoughertyJ... | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:BrownE LEL | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:CollinsMM | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:MeyersR ERE | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:KremskyJ NJN | lld:pubmed |
| pubmed-article:3562241 | pubmed:author | pubmed-author:WootersJ LJL | lld:pubmed |
| pubmed-article:3562241 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3562241 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:3562241 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:3562241 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3562241 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3562241 | pubmed:pagination | 2891-909 | lld:pubmed |
| pubmed-article:3562241 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:3562241 | pubmed:meshHeading | pubmed-meshheading:3562241-... | lld:pubmed |
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| pubmed-article:3562241 | pubmed:meshHeading | pubmed-meshheading:3562241-... | lld:pubmed |
| pubmed-article:3562241 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3562241 | pubmed:articleTitle | Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus. | lld:pubmed |
| pubmed-article:3562241 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3562241 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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