| pubmed-article:3539147 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3539147 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:3539147 | lifeskim:mentions | umls-concept:C0795635 | lld:lifeskim |
| pubmed-article:3539147 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:3539147 | lifeskim:mentions | umls-concept:C1579433 | lld:lifeskim |
| pubmed-article:3539147 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:3539147 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:3539147 | pubmed:dateCreated | 1987-2-4 | lld:pubmed |
| pubmed-article:3539147 | pubmed:abstractText | Replacement of B25-phenylalanine by leucine in the insulin sequence causes marked inactivation. The effect of this sequence variation was studied here in des-(B26-30)-insulin. [LeuB25]des-(B26-30)-insulin and its B25-amide were prepared by trypsin-mediated semisynthesis from N-terminally protected des-(B23-30)-insulin and synthetic tripeptides. The relative lipogenic potency in isolated rat adipocytes was 8.0% for the truncated analogue with a free B25-carboxyl function, and 18.1% for the amidated analogue. Binding to cultured human IM-9 lymphocytes was 4% and 9%, respectively. Thus, both shortened insulins are markedly more active than [LeuB25]insulin. The PheB25----LeuB25 substitution in both the shortened and the full sequence has a moderate effect on the CD spectrum, indicating that the gross main chain conformation is largely retained in both molecules. Independent of the substitution an absolute increase of the circular dichroism is observed upon amidation of the B25-carboxyl group. | lld:pubmed |
| pubmed-article:3539147 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3539147 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3539147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3539147 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3539147 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3539147 | pubmed:issn | 0177-3593 | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:De MeytsPP | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:WollmerAA | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:BrandenburgDD | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:ZahnHH | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:DiaconescuCC | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:SaundersDD | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:DodsonGG | lld:pubmed |
| pubmed-article:3539147 | pubmed:author | pubmed-author:FischerW HWH | lld:pubmed |
| pubmed-article:3539147 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3539147 | pubmed:volume | 367 | lld:pubmed |
| pubmed-article:3539147 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3539147 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3539147 | pubmed:pagination | 999-1006 | lld:pubmed |
| pubmed-article:3539147 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:meshHeading | pubmed-meshheading:3539147-... | lld:pubmed |
| pubmed-article:3539147 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3539147 | pubmed:articleTitle | Structure-function relationships of shortened [LeuB25]insulins, semisynthetic analogues of a mutant human insulin. | lld:pubmed |
| pubmed-article:3539147 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3539147 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
