| pubmed-article:3533916 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C0220806 | lld:lifeskim |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C0392918 | lld:lifeskim |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C1956015 | lld:lifeskim |
| pubmed-article:3533916 | lifeskim:mentions | umls-concept:C1522605 | lld:lifeskim |
| pubmed-article:3533916 | pubmed:issue | 30 | lld:pubmed |
| pubmed-article:3533916 | pubmed:dateCreated | 1986-12-1 | lld:pubmed |
| pubmed-article:3533916 | pubmed:abstractText | Protein-protein cross-linking was used to examine the spatial arrangement of proteins within the 40 S ribosomal subunits of Saccharomyces cerevisiae. Purified ribosomal subunits were treated with either 2-iminothiolane or dimethyl 3,3'-dithiobispropionimidate under conditions such that the ribosomal particle was intact and that formation of 40 S subunit dimers was minimized. Proteins were extracted from the treated subunits and fractionated on Sephadex G-150 or by acid-urea-polyacrylamide gel electrophoresis. Cross-linked proteins in these fractions were analyzed by two-dimensional diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Constituent members of cross-linked pairs were radiolabeled with 125I and identified by two-dimensional gel electrophoresis and comparison with nonradioactive ribosomal protein markers. Forty-two pairs involving 25 of the 32 40 S subunit proteins were identified. Many proteins were detected in several cross-linked dimers. These proteins with multiple cross-links form foci for the construction of a schematic model of the spatial arrangement of proteins within the 40 S subunit. | lld:pubmed |
| pubmed-article:3533916 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3533916 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3533916 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3533916 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3533916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3533916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3533916 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3533916 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3533916 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3533916 | pubmed:author | pubmed-author:LeeJ CJC | lld:pubmed |
| pubmed-article:3533916 | pubmed:author | pubmed-author:TrautR RRR | lld:pubmed |
| pubmed-article:3533916 | pubmed:author | pubmed-author:YehY CYC | lld:pubmed |
| pubmed-article:3533916 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3533916 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:3533916 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:3533916 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3533916 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3533916 | pubmed:pagination | 14148-53 | lld:pubmed |
| pubmed-article:3533916 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:meshHeading | pubmed-meshheading:3533916-... | lld:pubmed |
| pubmed-article:3533916 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3533916 | pubmed:articleTitle | Protein topography of the 40 S ribosomal subunit from Saccharomyces cerevisiae as shown by chemical cross-linking. | lld:pubmed |
| pubmed-article:3533916 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3533916 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3533916 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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