| pubmed-article:3520 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C1417825 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:3520 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:3520 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3520 | pubmed:dateCreated | 1976-5-18 | lld:pubmed |
| pubmed-article:3520 | pubmed:abstractText | It is generally assumed that hepatic transport of bile acids is a carrier-mediated process. However, the basic mechanisms by which these organic anions are translocated across the liver cell surface membrane are not well understood. Since carrier-mediated transport involved binding of the transported molecule to specific receptor sites, we have investigated the possibility that bile acid receptors are present in liver surface membranes. Isolated liver surface membranes were incubated at 4 degrees C with [14C]cholic acid and [14C]taurocholic acid, and membrane-boudn bile acid was separated from free by a rapid ultrafiltration technique through glass-fiber filters. Specific bile acid binding is rapid and reversible and represents approximately 80% of the total bile acid bound to liver surface membranes. Taurocholic acid binding is independent of the medium pH, while cholic acid binding demonstrates an optimum at pH 6.0. Analysis of equilibrium data for both cholic and taurocholic acid binding indicates that specific binding is saturable and consistent with Michaelis-Menten kinetics, while nonspecific binding is nonsaturable. Apparent maximal binding capacity and dissociation constant values indicate a large capacity system of receptors that have an affinity for bile acids comparable to that of the hepatic transport mechanism. Scatchard analysis of the saturation kinetics as well as inhibition studies suggest that bile acids bind to a single and noninteracting class of anion that competes with bile acids for hepatic uptake, also inhibits cholic acid binding. In contrast, no inhibition was demonstrated with indocyanine green and probenecid. Specific bile acid binding is enriched and primarily located in liver surface membranes and found only in tissues involved in bile acid transport. Specific bile acid binding is independnet of Na+, Ca2+, and Mg2+ and does not require metabolic energy. In addition, thiol groups and disulfide are not required for activity at the binding site. However, specific bile acid binding is markedly decreased by low concentrations of proteolytic enzymes and is also decreased by the action of neuraminidase and phospholipases A and C. These results are consistent with the existence of a homogeneous bile acid receptor protein in liver surface membranes. The primary surface membrane location of this receptor, its binding properties, and its ligand specificity suggest that bile acid binding to this receptor may represent the initial interaction in bile acid transport across liver surface membranes. | lld:pubmed |
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| pubmed-article:3520 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3520 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3520 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:3520 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3520 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:3520 | pubmed:issn | 0021-9738 | lld:pubmed |
| pubmed-article:3520 | pubmed:author | pubmed-author:SimonF RFR | lld:pubmed |
| pubmed-article:3520 | pubmed:author | pubmed-author:AccatinoLL | lld:pubmed |
| pubmed-article:3520 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3520 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:3520 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3520 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3520 | pubmed:pagination | 496-508 | lld:pubmed |
| pubmed-article:3520 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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