| pubmed-article:3499899 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3499899 | lifeskim:mentions | umls-concept:C0008778 | lld:lifeskim |
| pubmed-article:3499899 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:3499899 | lifeskim:mentions | umls-concept:C0085337 | lld:lifeskim |
| pubmed-article:3499899 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:3499899 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3499899 | pubmed:dateCreated | 1987-11-27 | lld:pubmed |
| pubmed-article:3499899 | pubmed:abstractText | The kinetics of the ionic regulation of an adenylate cyclase associated with the excitable ciliary membrane from Paramecium tetraurelia was examined. Glycerol (30%, v/v) stabilized the enzyme, and activated by an increase in Vmax. (3-fold) and a decrease in the apparent Km for MgATP (6-fold). Kinetic analysis of Mg2+ effects showed a stimulation via a single metal-binding site separate from the substrate site, with a dissociation constant, Ks, of 0.27 mM. Analysis of Ca2+ effects showed (i) an uncompetitive inhibition with respect to substrate MgATP, and (ii) dependence of the extent of inhibition on the free Mg2+ concentration. Ki values ranged from 4 to 130 microM-Ca2+ in the presence of 0.55-2 mM-Mg2+ respectively. This indicates competition between Mg2+ and Ca2+ at the metal-binding site. The Ca2+ effect was specific; Sr2+ and Ba2+ were almost without effect, and 100 microM-Ba2+ did not interfere with the Ca2+ inhibition. The actions of Ca2+ were readily reversible after addition of EGTA. K+ activated the adenylate cyclase at concentrations around 20 mM. The stimulatory potency of K+ was dependent on the free Mg2+ concentration. At 1 mM free Mg2+, 20 mM-K+ doubled the adenylate cyclase activity. The inhibitory Ca2+ and stimulatory K+ inputs were independent of each other. | lld:pubmed |
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| pubmed-article:3499899 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3499899 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3499899 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3499899 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3499899 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3499899 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:3499899 | pubmed:author | pubmed-author:KlumppSS | lld:pubmed |
| pubmed-article:3499899 | pubmed:author | pubmed-author:SchultzJ EJE | lld:pubmed |
| pubmed-article:3499899 | pubmed:author | pubmed-author:WanK KKK | lld:pubmed |
| pubmed-article:3499899 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3499899 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3499899 | pubmed:volume | 246 | lld:pubmed |
| pubmed-article:3499899 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3499899 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3499899 | pubmed:pagination | 187-92 | lld:pubmed |
| pubmed-article:3499899 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3499899 | pubmed:meshHeading | pubmed-meshheading:3499899-... | lld:pubmed |
| pubmed-article:3499899 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3499899 | pubmed:articleTitle | Ionic regulation of adenylate cyclase from the cilia of Paramecium tetraurelia. | lld:pubmed |
| pubmed-article:3499899 | pubmed:affiliation | Pharmazeutisches Institut der Universität, Tübingen, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:3499899 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3499899 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
