| pubmed-article:3492504 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C1136335 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C0178666 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C0031586 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C1554078 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:3492504 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
| pubmed-article:3492504 | pubmed:dateCreated | 1987-2-25 | lld:pubmed |
| pubmed-article:3492504 | pubmed:abstractText | Interleukin 3 (IL-3) promotes the survival, proliferation and development of progenitor cells from several distinct haemopoietic lineages and can also stimulate the self-renewal of stem cells. We have explored the mode of action of this growth factor in promoting survival and proliferation, using a multipotent haemopoietic stem cell line FDC-Mix 1. In the absence of IL-3 these cells died within 16-48 h. However, this requirement for IL-3 could be replaced by 12-O-tetradecanoylphorbol-13-acetate (TPA) plus Ca2+ ionophore, which promoted not only survival but also DNA synthesis with no concomitant loss of the multipotential nature of these cells. TPA and Ca2+ ionophore, respectively, could also interact synergistically with IL-3 to promote DNA synthesis. Both IL-3 and TPA stimulated the translocation of protein kinase C (PK-C) from the cytosol to a membrane-bound form in FDC-Mix 1 cells. Previously we suggested that IL-3 can activate the primary metabolism of IL-3-dependent cells so that increased glucose transport and glycolysis lead to maintenance of ATP levels and cellular survival. To investigate whether TPA and, or, Ca2+ ionophore could also influence cellular survival via an activation of glucose uptake we assessed the effects of these agents on hexose transport. TPA +/- Ca2+ ionophore activated hexose transport to the same degree as does IL-3 but these agents cannot superstimulate FDC-Mix 1 hexose transport in cells that already exhibit an activated transport system from preincubation with IL-3. We conclude that IL-3 maintains FDC-Mix 1 cells via its ability to activate PK-C and increase cytosolic levels of Ca2+, and that an IL-3-mediated activation of PK-C may promote cellular survival via its ability to enhance hexose uptake by phosphorylating the glucose transport protein. | lld:pubmed |
| pubmed-article:3492504 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3492504 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3492504 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3492504 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3492504 | pubmed:issn | 0021-9533 | lld:pubmed |
| pubmed-article:3492504 | pubmed:author | pubmed-author:DexterT MTM | lld:pubmed |
| pubmed-article:3492504 | pubmed:author | pubmed-author:HeyworthC MCM | lld:pubmed |
| pubmed-article:3492504 | pubmed:author | pubmed-author:WhettonA DAD | lld:pubmed |
| pubmed-article:3492504 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3492504 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:3492504 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3492504 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3492504 | pubmed:pagination | 93-104 | lld:pubmed |
| pubmed-article:3492504 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
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| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:meshHeading | pubmed-meshheading:3492504-... | lld:pubmed |
| pubmed-article:3492504 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3492504 | pubmed:articleTitle | Phorbol esters activate protein kinase C and glucose transport and can replace the requirement for growth factor in interleukin-3-dependent multipotent stem cells. | lld:pubmed |
| pubmed-article:3492504 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3492504 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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