| pubmed-article:3474231 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C0023418 | lld:lifeskim |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C1512310 | lld:lifeskim |
| pubmed-article:3474231 | lifeskim:mentions | umls-concept:C0048819 | lld:lifeskim |
| pubmed-article:3474231 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:3474231 | pubmed:dateCreated | 1987-8-13 | lld:pubmed |
| pubmed-article:3474231 | pubmed:abstractText | The influx of 5'-deoxy-5'-methylthioadenosine (MeSAdo) into human HL-60 leukemia cells and erythrocytes was characterized in order to determine whether it is facilitated by the nonspecific nucleoside carrier system or by a separate transporter, as suggested by other reports. Initial velocities were measured at room temperature by means of inhibitor-stop and oil-stop assays. MeSAdo influx was strongly inhibited by Ado, dAdo, and nucleoside transport inhibitors including nitrobenzylthioinosine and dipyridamole. Ade was inhibitory only at concentrations in excess of 1 mM. Loss of nucleoside transport capacity during differentiation of HL-60 cells was accompanied by a corresponding decrease in MeSAdo influx rates. These results indicate that MeSAdo influx was mediated by the nonspecific nucleoside transport system. The kinetic data were consistent with a single saturable carrier and yielded Km values of 74 and 184 microM and Vmax values of 424 and 48 pmols/10(6) cells/min with HL-60 cells and erythrocytes, respectively, after correction for a substantial passive diffusion component, which accounted for over 50% of the influx of 1 mM MeSAdo. The passive diffusion of MeSAdo in the presence of a transport inhibitor was not rate-limiting for the salvage of 50 microM MeSAdo to methionine when HL-60 cells were cultured in methionine-deficient medium. The large contribution of passive diffusion to the influx of MeSAdo is consistent with its unusually high octanol/water partition ratio (5.7-fold greater than that of Ado). | lld:pubmed |
| pubmed-article:3474231 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3474231 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3474231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3474231 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3474231 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3474231 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3474231 | pubmed:author | pubmed-author:StoecklerJ... | lld:pubmed |
| pubmed-article:3474231 | pubmed:author | pubmed-author:LiS YSY | lld:pubmed |
| pubmed-article:3474231 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3474231 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:3474231 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:3474231 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3474231 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3474231 | pubmed:pagination | 9542-6 | lld:pubmed |
| pubmed-article:3474231 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:meshHeading | pubmed-meshheading:3474231-... | lld:pubmed |
| pubmed-article:3474231 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3474231 | pubmed:articleTitle | Influx of 5'-deoxy-5'-methylthioadenosine into HL-60 human leukemia cells and erythrocytes. | lld:pubmed |
| pubmed-article:3474231 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3474231 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3474231 | lld:pubmed |
