| pubmed-article:3467359 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:3467359 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:3467359 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:3467359 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:3467359 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:3467359 | lifeskim:mentions | umls-concept:C0086149 | lld:lifeskim |
| pubmed-article:3467359 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3467359 | pubmed:dateCreated | 1987-2-18 | lld:pubmed |
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| pubmed-article:3467359 | pubmed:abstractText | cDNA clones encoding the regulatory subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) from Dictyostelium discoideum were isolated by immunoscreening of a cDNA library constructed in the expression vector lambda gt11. High-affinity cAMP-binding activity was detected in extracts from bacteria lysogenized with these clones. Nucleotide sequence analysis of three overlapping clones allowed the determination of a 1195-base-pair cDNA sequence coding for the entire regulatory subunit and containing nontranslated 5' and 3' sequences. The open reading frame codes for a protein of 327 amino acids, with molecular weight 36,794. The regulatory subunit from Dictyostelium shares a high degree of homology with its mammalian counterparts, but is lacking the NH2-terminal domain required for the association of regulatory subunits into dimers in other eukaryotes. On the basis of the comparison of the regulatory subunits from Dictyostelium, yeast, and bovine tissues, a model for the evolution of these proteins is proposed. | lld:pubmed |
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| pubmed-article:3467359 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3467359 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3467359 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3467359 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3467359 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:3467359 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3467359 | pubmed:author | pubmed-author:LacombeM LML | lld:pubmed |
| pubmed-article:3467359 | pubmed:author | pubmed-author:VeronMM | lld:pubmed |
| pubmed-article:3467359 | pubmed:author | pubmed-author:SimonM NMN | lld:pubmed |
| pubmed-article:3467359 | pubmed:author | pubmed-author:de GunzburgJJ | lld:pubmed |
| pubmed-article:3467359 | pubmed:author | pubmed-author:MutzelRR | lld:pubmed |
| pubmed-article:3467359 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3467359 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:3467359 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3467359 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3467359 | pubmed:pagination | 6-10 | lld:pubmed |
| pubmed-article:3467359 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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| pubmed-article:3467359 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3467359 | pubmed:articleTitle | Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum. | lld:pubmed |
| pubmed-article:3467359 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3467359 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3467359 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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