| pubmed-article:3462707 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0024880 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0282547 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0021764 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:3462707 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:3462707 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:3462707 | pubmed:dateCreated | 1986-10-3 | lld:pubmed |
| pubmed-article:3462707 | pubmed:abstractText | The heparin-containing mast cells that reside in the connective tissue of the mouse, but not the chondroitin sulfate-containing mast cells in the gastrointestinal mucosa, stain with safranin when exposed to alcian blue/safranin. Mouse bone marrow-derived mast cells (BMMC), the probable in vitro counterparts of in vivo mucosal mast cells, were cultured for 14 days with mouse skin-derived 3T3 fibroblasts in RPMI 1640 medium containing 10% fetal calf serum and 50% WEHI-3 conditioned medium. Although the BMMC adhered to the fibroblast monolayer, they continued to divide, probably due to the presence of interleukin 3 in the conditioned medium. The mast cells remained viable throughout the period of coculture, since they failed to release lactate dehydrogenase and because they increased their histamine content approximately 15-fold. After 12-14 days of coculture, greater than 50% of the BMMC changed histochemically to become safranin+; 30-40% of the 35S-labeled glycosaminoglycans on the proteoglycans synthesized by these cocultured mast cells were heparin, whereas heparin was not detected in the initial BMMC. In the absence of WEHI-3 conditioned medium, BMMC adhered to the fibroblast monolayer, and after 8 days of coculture, the number of mast cells did not change and their histamine content remained the same. However, these mast cells also became safranin+ and synthesized 40% heparin glycosaminoglycans. Thus, coculture of BMMC with fibroblasts induces a phenotypic change so that the resulting mast cells stain safranin+ and synthesize heparin proteoglycans, whereas the presence of WEHI-3 conditioned medium stimulates proliferation and an increase in histamine content. | lld:pubmed |
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| pubmed-article:3462707 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3462707 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3462707 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3462707 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3462707 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3462707 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3462707 | pubmed:author | pubmed-author:AustenK FKF | lld:pubmed |
| pubmed-article:3462707 | pubmed:author | pubmed-author:StevensR LRL | lld:pubmed |
| pubmed-article:3462707 | pubmed:author | pubmed-author:Levi-Schaffer... | lld:pubmed |
| pubmed-article:3462707 | pubmed:author | pubmed-author:GravalleseP... | lld:pubmed |
| pubmed-article:3462707 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3462707 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:3462707 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3462707 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3462707 | pubmed:pagination | 6485-8 | lld:pubmed |
| pubmed-article:3462707 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3462707 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3462707 | pubmed:articleTitle | Coculture of interleukin 3-dependent mouse mast cells with fibroblasts results in a phenotypic change of the mast cells. | lld:pubmed |
| pubmed-article:3462707 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3462707 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3462707 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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