| pubmed-article:3408791 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C1706515 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C0079720 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C1704640 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C0596751 | lld:lifeskim |
| pubmed-article:3408791 | lifeskim:mentions | umls-concept:C0331858 | lld:lifeskim |
| pubmed-article:3408791 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3408791 | pubmed:dateCreated | 1988-10-3 | lld:pubmed |
| pubmed-article:3408791 | pubmed:abstractText | New polymeric solid-phase matrices for cell affinity chromatography were prepared and their advantageous characteristics compared with conventional matrices were highlighted. These new matrices are derivatives of poly(2-hydroxyethyl methacrylate) (PHEMA) containing a slight quantity of amino compounds as a co-monomer. They were applied to immunoaffinity selection between IgG+ and IgG- lymphocytes of the rat mesenteric lymph node. Simple physical adsorption was sufficient for anti-rat IgG antibodies to be immobilized on these matrices, allowing us to omit the laborious procedure of covalent-linking of antibodies on a matrix. As these matrices themselves showed extremely low non-specific adsorption of lymphocytes, a very dilute solution of antibody (0.02-0.08 mg/ml) was enough for column conditioning. This separation method gave IgG- lymphocytes of more than 90% purity and almost 95% yield within as short a time as 7 min. Further, IgG+ lymphocytes were obtained in good yield (80-90% of loaded number) by recovering the adsorbing cell fraction from the column by gentle pipetting of the matrix. | lld:pubmed |
| pubmed-article:3408791 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3408791 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3408791 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3408791 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3408791 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3408791 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3408791 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3408791 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3408791 | pubmed:month | May | lld:pubmed |
| pubmed-article:3408791 | pubmed:issn | 0142-9612 | lld:pubmed |
| pubmed-article:3408791 | pubmed:author | pubmed-author:KataokaKK | lld:pubmed |
| pubmed-article:3408791 | pubmed:author | pubmed-author:SakuraiYY | lld:pubmed |
| pubmed-article:3408791 | pubmed:author | pubmed-author:TsurutaTT | lld:pubmed |
| pubmed-article:3408791 | pubmed:author | pubmed-author:MaruyamaAA | lld:pubmed |
| pubmed-article:3408791 | pubmed:author | pubmed-author:HanaiTT | lld:pubmed |
| pubmed-article:3408791 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3408791 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:3408791 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3408791 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3408791 | pubmed:pagination | 218-24 | lld:pubmed |
| pubmed-article:3408791 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:meshHeading | pubmed-meshheading:3408791-... | lld:pubmed |
| pubmed-article:3408791 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3408791 | pubmed:articleTitle | Immunoaffinity chromatography of lymphocyte subpopulations using tert-amine derived matrices with adsorbed antibodies. | lld:pubmed |
| pubmed-article:3408791 | pubmed:affiliation | Institute of Biomedical Engineering, Tokyo Women's Medical College, Japan. | lld:pubmed |
| pubmed-article:3408791 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3408791 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
