| pubmed-article:3392674 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0029418 | lld:lifeskim |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0037473 | lld:lifeskim |
| pubmed-article:3392674 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:3392674 | pubmed:dateCreated | 1988-8-24 | lld:pubmed |
| pubmed-article:3392674 | pubmed:abstractText | 1. The whole-cell voltage-clamp mode of the patch-clamp technique was used to investigate the presence of voltage-gated inward currents in osteoblasts from newborn rat calvaria. 2. In K+-free solutions, three kinds of inward currents could be activated by depolarization: a voltage-gated Na+ current and two different types of Ca2+ currents. 3. The Na+ current was activated by depolarization above -40 mV in all the cells. It was reduced by half by 10 nM-TTX (tetrodotoxin). 4. In an isotonic Ba2+ external solution containing TTX, and with a Cs-EGTA internal solution buffered at pCa 8, depolarizing jumps induced both a transient Ba2+ current and a sustained Ba2+ current. The relative proportions of these two currents varied greatly among cells. 5. The transient and sustained Ba2+ currents differ with respect to their time course and their voltage dependence. 6. The depolarization-activated inward currents were also observed under more physiological conditions, in the presence of only 2 mM-external Ca2+ and with a K+ internal solution buffered at pCa 7. 7. A few records obtained in current clamp showed that it is possible to induce action potentials in osteoblasts. | lld:pubmed |
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| pubmed-article:3392674 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3392674 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3392674 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3392674 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3392674 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3392674 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:3392674 | pubmed:author | pubmed-author:FritschJJ | lld:pubmed |
| pubmed-article:3392674 | pubmed:author | pubmed-author:Chesnoy-March... | lld:pubmed |
| pubmed-article:3392674 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3392674 | pubmed:volume | 398 | lld:pubmed |
| pubmed-article:3392674 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3392674 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3392674 | pubmed:pagination | 291-311 | lld:pubmed |
| pubmed-article:3392674 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3392674 | pubmed:meshHeading | pubmed-meshheading:3392674-... | lld:pubmed |
| pubmed-article:3392674 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3392674 | pubmed:articleTitle | Voltage-gated sodium and calcium currents in rat osteoblasts. | lld:pubmed |
| pubmed-article:3392674 | pubmed:affiliation | Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France. | lld:pubmed |
| pubmed-article:3392674 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3392674 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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