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pubmed-article:3340714pubmed:abstractTextThe effect of oxygen on cell cycle delay by low doses of radiation on synchronized Harding Passey melanoma cells has been studied. Cells were irradiated 5 h after subculturing into fresh medium, and the delay before the start of S was measured. DNA synthesis was measured by frequent pulse labeling of the cells with radioactive thymidine to obtain the S-phase profile. The amount by which the irradiated cells S-phase profile had to be moved in time so that both the ascending and descending portions of the first S phase overlayed that of the controls was used as a measure of the delay. The magnitude of the delay was exponentially related to radiation dose and the effect of irradiating in the absence of oxygen was a dose multiplying factor of 2.5. This was similar to the oxygen effect on survival for cells irradiated under the same conditions.lld:pubmed
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pubmed-article:3340714pubmed:authorpubmed-author:DeweyD LDLlld:pubmed
pubmed-article:3340714pubmed:authorpubmed-author:WilliamsK BKBlld:pubmed
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pubmed-article:3340714pubmed:volume113lld:pubmed
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pubmed-article:3340714pubmed:pagination102-11lld:pubmed
pubmed-article:3340714pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3340714pubmed:year1988lld:pubmed
pubmed-article:3340714pubmed:articleTitleThe effect of oxygen on the delay in the initiation of DNA synthesis after irradiation.lld:pubmed
pubmed-article:3340714pubmed:affiliationCancer Research Campaign, Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, England.lld:pubmed
pubmed-article:3340714pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3340714pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed