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pubmed-article:3264007pubmed:abstractTextGangliosides have been prepared from resting murine thymocytes and splenic T cells. Profoundly different two-dimensional thin layer chromatography (2D TLC) patterns were observed between these two cell types. Thymocytes contained 28-30 discrete gangliosides of which eight represented major gangliosides. Splenic T lymphocytes from both strains had much simpler patterns, with six to seven major gangliosides and 12-13 minor gangliosides. Computerized analysis of the thymocyte ganglioside patterns between LPS-responder C3H/HeN mice and lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice revealed no significant difference in the major gangliosides. However, with splenic T cell gangliosides, there is a striking difference in the relative proportion of three homologous gangliosides between the two strains. Consistent with previous observations on macrophage gangliosides, the ratio of N-acetylneuraminic acid-containing ganglioside to N-glycolylneuraminic acid-containing ganglioside was higher in both thymocytes and T-cells from the LPS-responder strain. These results show that sialic acid-containing glycolipids from thymocytes and T lymphocytes between endotoxin responder and hyporesponder strains manifest small but significant changes. These differences are present in unstimulated cell populations and may represent a manifestation of the Lps gene.lld:pubmed
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pubmed-article:3264007pubmed:authorpubmed-author:RyanJ LJLlld:pubmed
pubmed-article:3264007pubmed:authorpubmed-author:YoheH CHClld:pubmed
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pubmed-article:3264007pubmed:pagination521-8lld:pubmed
pubmed-article:3264007pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:3264007pubmed:articleTitleComparison of thymocyte and T lymphocyte gangliosides from C3H/HeN and C3H/HeJ mice.lld:pubmed
pubmed-article:3264007pubmed:affiliationDepartment of Internal Medicine, Yale University School of Medicine, West Haven, CT 06516.lld:pubmed
pubmed-article:3264007pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3264007pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:3264007pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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