| pubmed-article:3259140 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C0019692 | lld:lifeskim |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:3259140 | lifeskim:mentions | umls-concept:C0302891 | lld:lifeskim |
| pubmed-article:3259140 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3259140 | pubmed:dateCreated | 1988-6-23 | lld:pubmed |
| pubmed-article:3259140 | pubmed:abstractText | We partially purified the human immunodeficiency virus (HIV) glycoprotein gp41 from infected H9 cells by immunoaffinity chromatography using a column containing the M25 monoclonal antibody (diMarzo-Veronese et al., 1985). A pH 11.5 buffer worked best for eluting the glycoprotein from this column. The eluted gp41 was used in a sensitive slot blot immunoassay to detect antibodies to HIV in human sera and to prepare rabbit polyclonal antibodies and the 41-1S mouse monoclonal antibody. These antibodies reacted with gp41 in immunoprecipitation and in Western blot assays, but did not neutralize HIV in a syncytium-forming microassay. A pH 2.5 buffer was found to be the most effective solution for eluting gp41 from a 41-1S monoclonal antibody column. | lld:pubmed |
| pubmed-article:3259140 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3259140 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3259140 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3259140 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:3259140 | pubmed:issn | 0889-2229 | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:ZweigMM | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:FischingerP... | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:RobeyW GWG | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:GildenR VRV | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:ArthurL OLO | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:ShowalterS... | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:NaraP LPL | lld:pubmed |
| pubmed-article:3259140 | pubmed:author | pubmed-author:BladenS VSV | lld:pubmed |
| pubmed-article:3259140 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3259140 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:3259140 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3259140 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3259140 | pubmed:pagination | 51-62 | lld:pubmed |
| pubmed-article:3259140 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:meshHeading | pubmed-meshheading:3259140-... | lld:pubmed |
| pubmed-article:3259140 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3259140 | pubmed:articleTitle | Partial purification of native HIV transmembrane protein gp41: generation of polyclonal and monoclonal antibodies. | lld:pubmed |
| pubmed-article:3259140 | pubmed:affiliation | Program Resources, Inc., NCI-Frederick Cancer Research Facility, MD. | lld:pubmed |
| pubmed-article:3259140 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3259140 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3259140 | lld:pubmed |
