| pubmed-article:3239750 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0442034 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0023779 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0014994 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C0032172 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:3239750 | lifeskim:mentions | umls-concept:C1511572 | lld:lifeskim |
| pubmed-article:3239750 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3239750 | pubmed:dateCreated | 1989-5-5 | lld:pubmed |
| pubmed-article:3239750 | pubmed:abstractText | Cell-free preparations of ionophore-stimulated peritoneal rat polymorphonuclear neutrophils (PMNs) incubated with 1-(N-dansyl-11-amino-1-undecyl)-sn-glycerol-3-phosphorylcholine (dansyllyso-PAF) converted this fluorescent lyso ether lipid into two different classes of products. In the absence of acetyl-CoA 1-(N-dansyl-11-amino-1-undecyl)-2-long chain acyl-sn-glycerol-3-phosphorylcholine (dansylalkyl-2-acyl-GPC) was the only identified new fluorescent phospholipid. In the presence of acetyl-CoA an additional new product, 1-(N-dansyl-11-amino-1-undecyl)-2-acetyl-sn-glycerol-3-phosphorylcholine (dansyl-PAF), was formed. The formation of dansyl-PAF in PMN homogenates was only transient with a maximum after about 4 min. When PMN homogenates were incubated with dansyl-PAF the formation of dansyllyso-PAF was observed prior to the formation of dansyl-2-acyl-GPC. Thus, our data indicate that enzymatically formed dansyl-PAF is completely remodeled into dansylalkyl-2-acyl-GPC by the sequential action of PAF acetylhydrolase and CoA-independent transacylase. These results demonstrate that peritoneal rat PMNs contain lyso-PAF acetyltransferase, PAF acetylhydrolase, and CoA-independent transacylase and that fluorophore-labeled ether lipids provide an easy means to assay enzymes which catalyze important enzymatic reactions involved in the biosynthesis and remodeling of platelet-activating factor. | lld:pubmed |
| pubmed-article:3239750 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3239750 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3239750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3239750 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3239750 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:3239750 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:3239750 | pubmed:author | pubmed-author:WalterRR | lld:pubmed |
| pubmed-article:3239750 | pubmed:author | pubmed-author:HendricksonH... | lld:pubmed |
| pubmed-article:3239750 | pubmed:author | pubmed-author:SchindlerP... | lld:pubmed |
| pubmed-article:3239750 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3239750 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:3239750 | pubmed:volume | 174 | lld:pubmed |
| pubmed-article:3239750 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3239750 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3239750 | pubmed:pagination | 477-84 | lld:pubmed |
| pubmed-article:3239750 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:meshHeading | pubmed-meshheading:3239750-... | lld:pubmed |
| pubmed-article:3239750 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3239750 | pubmed:articleTitle | Fluorophore-labeled ether lipids: substrates for enzymes of the platelet-activating factor cycle in peritoneal polymorphonuclear leukocytes. | lld:pubmed |
| pubmed-article:3239750 | pubmed:affiliation | Pharma Forschung, Hoechst AG, Frankfurt, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:3239750 | pubmed:publicationType | Journal Article | lld:pubmed |
