pubmed-article:3235044 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:3235044 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
pubmed-article:3235044 | lifeskim:mentions | umls-concept:C0682570 | lld:lifeskim |
pubmed-article:3235044 | lifeskim:mentions | umls-concept:C0037868 | lld:lifeskim |
pubmed-article:3235044 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:3235044 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:3235044 | pubmed:dateCreated | 1989-4-27 | lld:pubmed |
pubmed-article:3235044 | pubmed:abstractText | A purified head fraction was prepared from bovine epididymal spermatozoa and was utilized to identify the solubility characteristics and major polypeptide components of the postacrosomal sheath. Sperm heads extracted in nonionic-detergent-containing or high-salt-containing solutions retained an intact postacrosomal sheath, but it was readily solubilized by high pH extraction solutions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a major polypeptide of 58,000 daltons (58-kD) in the high pH extract solution. Antibodies to the 58-kD polypeptide specifically reacted with the postacrosomal segment by immunofluorescence and by electron microscopic immunohistochemistry were shown to bind the postacrosomal sheath. We conclude that this 58-kD polypeptide is a constituent of the postacrosomal sheath and that its distribution is restricted to the postacrosomal segment. | lld:pubmed |
pubmed-article:3235044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3235044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3235044 | pubmed:language | eng | lld:pubmed |
pubmed-article:3235044 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3235044 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:3235044 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:3235044 | pubmed:month | Jul | lld:pubmed |
pubmed-article:3235044 | pubmed:issn | 0148-7280 | lld:pubmed |
pubmed-article:3235044 | pubmed:author | pubmed-author:OlsonG EGE | lld:pubmed |
pubmed-article:3235044 | pubmed:author | pubmed-author:WinfreyV PVP | lld:pubmed |
pubmed-article:3235044 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:3235044 | pubmed:volume | 20 | lld:pubmed |
pubmed-article:3235044 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:3235044 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:3235044 | pubmed:pagination | 329-42 | lld:pubmed |
pubmed-article:3235044 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
pubmed-article:3235044 | pubmed:meshHeading | pubmed-meshheading:3235044-... | lld:pubmed |
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pubmed-article:3235044 | pubmed:meshHeading | pubmed-meshheading:3235044-... | lld:pubmed |
pubmed-article:3235044 | pubmed:year | 1988 | lld:pubmed |
pubmed-article:3235044 | pubmed:articleTitle | Characterization of the postacrosomal sheath of bovine spermatozoa. | lld:pubmed |
pubmed-article:3235044 | pubmed:affiliation | Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232. | lld:pubmed |
pubmed-article:3235044 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:3235044 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:3235044 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |