| pubmed-article:3170599 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0027836 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C1882598 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C2612126 | lld:lifeskim |
| pubmed-article:3170599 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:3170599 | pubmed:issue | 30 | lld:pubmed |
| pubmed-article:3170599 | pubmed:dateCreated | 1988-11-21 | lld:pubmed |
| pubmed-article:3170599 | pubmed:abstractText | D-Glucose deprivation of primary rat brain glial cell cultures, by incubation with 25 mM D-fructose for 24 h, resulted in a 4-5-fold induction of D-glucose transport activity. In contrast, 24-h D-glucose starvation of primary rat brain neuronal cultures had only a marginal effect (1.5-2-fold) on D-glucose transport activity. Northern blot analysis of total cellular RNA demonstrated that under these conditions the rat brain glial cells specifically increased the steady-state level of the D-glucose transporter mRNA 4-6-fold, whereas Northern blot analysis of the neuronal cell cultures revealed no significant alteration in the amount of D-glucose transporter mRNA by D-glucose deprivation. These findings demonstrated that the D-glucose-dependent regulation of the D-glucose transporter system occurred in a brain cell type-specific manner. The ED50 for the D-glucose starvation increase in the D-glucose transporter mRNA, in the glial cell cultures, occurred at approximately 3.5 mM D-glucose with maximal effect at 0.5 mM D-glucose. Readdition of D-glucose to the starved cell cultures reversed the increase in the D-glucose transporter mRNA levels and D-glucose transport activity to control values within 24 h. The increase in the D-glucose transporter mRNA was relatively rapid with half-maximal stimulation at approximately 2 h and maximal induction by 6-12 h of D-glucose deprivation. A similar time course was also observed for the starvation-induced increase in D-glucose transport activity and D-glucose transporter protein, as determined by Western blot analysis. These results document that, in rat brain glial cells, D-glucose transport activity, protein, and mRNA are regulated by the extracellular D-glucose concentration. Further, this suggests a potential role for hyperglycemia in the down-regulation of the D-glucose transport system in vivo. | lld:pubmed |
| pubmed-article:3170599 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3170599 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3170599 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3170599 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3170599 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3170599 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3170599 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3170599 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3170599 | pubmed:author | pubmed-author:WalkerP SPS | lld:pubmed |
| pubmed-article:3170599 | pubmed:author | pubmed-author:FellowsR ERE | lld:pubmed |
| pubmed-article:3170599 | pubmed:author | pubmed-author:PessinJ EJE | lld:pubmed |
| pubmed-article:3170599 | pubmed:author | pubmed-author:Van NessB GBG | lld:pubmed |
| pubmed-article:3170599 | pubmed:author | pubmed-author:DonovanJ AJA | lld:pubmed |
| pubmed-article:3170599 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3170599 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:3170599 | pubmed:volume | 263 | lld:pubmed |
| pubmed-article:3170599 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3170599 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3170599 | pubmed:pagination | 15594-601 | lld:pubmed |
| pubmed-article:3170599 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3170599 | pubmed:meshHeading | pubmed-meshheading:3170599-... | lld:pubmed |
| pubmed-article:3170599 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3170599 | pubmed:articleTitle | Glucose-dependent regulation of glucose transport activity, protein, and mRNA in primary cultures of rat brain glial cells. | lld:pubmed |
| pubmed-article:3170599 | pubmed:affiliation | Department of Physiology and Biophysics, University of Iowa, Iowa City 52242. | lld:pubmed |
| pubmed-article:3170599 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3170599 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3170599 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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