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pubmed-article:3163811pubmed:abstractTextBilateral perifusion devices were utilized to measure prostaglandin secretion towards luminal and myometrial sides of bovine endometria. Tissues were collected at Day 17 post-estrus from cyclic (n = 4), pregnant (n = 5) and bred but subsequently non-pregnant (n = 6) cows. Tissue from each cow was placed into two perifusion devices, perifused with Krebs-Ringer Bicarbonate solution (3 ml/10 min) for 2.5 h and fractions collected every 10 min. Oxytocin (1 IU/ml) was perifused during fractions 7-12 to the luminal side of one device and to the myometrial side of the other device. Regardless of status, prostaglandin secretion rates (PGF and PGE2) were higher (P less than 0.01) from the luminal side than the myometrial side. Secretion rates of PGF were lower (P less than 0.01) for endometria from pregnant cows than for endometria from cyclic or bred/non-pregnant cows, whereas secretion rates of PGE2 were not affected by pregnancy status. Regardless of the side of perifusion, secretion rates of PGF and PGE2 from endometria of cyclic and bred/non-pregnant cows were elevated (P less than 0.01) throughout the period of oxytocin treatment, whereas prostaglandin secretion by endometria from pregnant cows was not stimulated by oxytocin. Decreased secretion of PGF from endometria of pregnant cows suggests that the corpus luteum and pregnancy are maintained because of an inhibition of endometrial prostaglandin synthesis or an inability to respond to stimulators of prostaglandin synthesis (i.e. oxytocin).lld:pubmed
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pubmed-article:3163811pubmed:authorpubmed-author:LacroixM CMClld:pubmed
pubmed-article:3163811pubmed:authorpubmed-author:ThatcherW WWWlld:pubmed
pubmed-article:3163811pubmed:authorpubmed-author:HansenP JPJlld:pubmed
pubmed-article:3163811pubmed:authorpubmed-author:GrossT STSlld:pubmed
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pubmed-article:3163811pubmed:volume35lld:pubmed
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pubmed-article:3163811pubmed:pagination343-57lld:pubmed
pubmed-article:3163811pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:3163811pubmed:year1988lld:pubmed
pubmed-article:3163811pubmed:articleTitleProstaglandin secretion by perifused bovine endometrium: secretion towards the myometrial and luminal sides at day 17 post-estrus as altered by pregnancy.lld:pubmed
pubmed-article:3163811pubmed:affiliationDepartment of Dairy Science, University of Florida, Gainesville 32611.lld:pubmed
pubmed-article:3163811pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3163811pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:3163811pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed