| pubmed-article:3148810 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C0376624 | lld:lifeskim |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C0074447 | lld:lifeskim |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C0148323 | lld:lifeskim |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C0205280 | lld:lifeskim |
| pubmed-article:3148810 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:3148810 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:3148810 | pubmed:dateCreated | 1989-4-27 | lld:pubmed |
| pubmed-article:3148810 | pubmed:abstractText | A cytotoxin to Vero cells (Shiga-like toxin), which was neutralized by antibody against purified Shiga toxin produced by Shigella dysenteriae 1, was purified from Escherichia coli O157:H7, isolated from a patient with hemorrhagic colitis. The purification procedure consisted of ammonium sulfate fractionation, DEAE-cellulose column chromatography, chromatofocusing column chromatography and high performance liquid chromatography. About 200 micrograms of purified Shiga-like toxin was obtained from cell extracts of 14 liters of culture with a yield of about 15%. The purified Shiga-like toxin showed identical physicochemical, biological and immunological properties to those of Shiga toxin. Purified Shiga-like toxin and Shiga toxin also had the same mobilities on polyacrylamide disc gel electrophoresis and polyacrylamide gel isoelectrofocusing. On sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, purified Shiga-like toxin migrated as two bands corresponding to the A and B subunits, and these migrated to the same positions as A and B subunits of Shiga toxin. The amino acid composition of the purified Shiga-like toxin was also similar to that of Shiga toxin. The purified Shiga-like toxin showed various biological activities: lethal toxicity to mice when injected intraperitoneally, the LD50 being 30 ng per mouse; cytotoxicity to Vero cells, killing about 50% of the cells at 6 pg; and fluid accumulation in rabbit ileal loops at concentrations of more than 1.25 micrograms/loop. These values are comparable with those obtained with Shiga toxin. In an Ouchterlony double gel diffusion test, the lines formed by the purified Shiga-like toxin and Shiga toxin fused, indicating that the two toxins were immunologically identical. | lld:pubmed |
| pubmed-article:3148810 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3148810 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3148810 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3148810 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3148810 | pubmed:month | May | lld:pubmed |
| pubmed-article:3148810 | pubmed:issn | 0882-4010 | lld:pubmed |
| pubmed-article:3148810 | pubmed:author | pubmed-author:TakedaYY | lld:pubmed |
| pubmed-article:3148810 | pubmed:author | pubmed-author:HirayamaTT | lld:pubmed |
| pubmed-article:3148810 | pubmed:author | pubmed-author:NodaMM | lld:pubmed |
| pubmed-article:3148810 | pubmed:author | pubmed-author:NakabayashiNN | lld:pubmed |
| pubmed-article:3148810 | pubmed:author | pubmed-author:YutsudoTT | lld:pubmed |
| pubmed-article:3148810 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3148810 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:3148810 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3148810 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3148810 | pubmed:pagination | 339-49 | lld:pubmed |
| pubmed-article:3148810 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:3148810 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3148810 | pubmed:articleTitle | Purification and some properties of Shiga-like toxin from Escherichia coli O157:H7 that is immunologically identical to Shiga toxin. | lld:pubmed |
| pubmed-article:3148810 | pubmed:affiliation | Department of Bacterial Infection, Institute of Medical Science, University of Tokyo, Japan. | lld:pubmed |
| pubmed-article:3148810 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3148810 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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