| pubmed-article:3145093 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3145093 | lifeskim:mentions | umls-concept:C1273518 | lld:lifeskim |
| pubmed-article:3145093 | lifeskim:mentions | umls-concept:C0442874 | lld:lifeskim |
| pubmed-article:3145093 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:3145093 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:3145093 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:3145093 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3145093 | pubmed:dateCreated | 1989-2-22 | lld:pubmed |
| pubmed-article:3145093 | pubmed:abstractText | The present studies were carried out to investigate the comparative roles of protein cross-linking and alteration in protein phosphorylation in the accumulation of neurofilaments due to aliphatic hexacarbons. In these studies, rats were given 2,5-hexanedione (0, 0.1, 0.25 and 1.0%) for 70 days in their drinking water. In a separate study of in vitro protein phosphorylation rats were given 1% 2,5-hexanedione for 14 days in their drinking water. Spinal cord neurofilaments were isolated and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting using anti-neurofilament antibodies, radioimmunoassays (RIAs) of phosphorylated epitopes on neurofilament proteins and protein phosphorylation. Protein cross-linking of neurofilaments was found in all animals treated with 2,5-hexanedione including the lowest dose (0.1%) which did not produce clinical signs of intoxication. Protein phosphorylation of neurofilament proteins, as well as MAP-2 was significantly decreased upon treatment. Protein staining revealed a decreased amount of neurofilament protein and immunoblotting demonstrated neurofilament protein cross-linking in these animals. Protein staining of glial fibrillary acidic protein (GFAP) was unaltered by this treatment. RIAs of phosphorylated and non-phosphorylated epitopes of neurofilament proteins indicated that in vivo phosphorylation of these proteins was also decreased. Two-dimensional gel electrophoresis indicated a shift of the neurofilament proteins to a basic pI, indicating a dephosphorylation of neurofilament proteins. Cross-linked neurofilament proteins also exhibited a pI which was more basic than any of the individual neurofilament proteins. This report demonstrates differential effects of 2,5-hexanedione on neurofilament proteins and indicates that several mechanisms may be responsible for their accumulation. | lld:pubmed |
| pubmed-article:3145093 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3145093 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3145093 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3145093 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3145093 | pubmed:issn | 0006-8993 | lld:pubmed |
| pubmed-article:3145093 | pubmed:author | pubmed-author:Abou-DoniaM... | lld:pubmed |
| pubmed-article:3145093 | pubmed:author | pubmed-author:LapadulaD MDM | lld:pubmed |
| pubmed-article:3145093 | pubmed:author | pubmed-author:SuwitaEE | lld:pubmed |
| pubmed-article:3145093 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3145093 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:3145093 | pubmed:volume | 458 | lld:pubmed |
| pubmed-article:3145093 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3145093 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3145093 | pubmed:pagination | 123-31 | lld:pubmed |
| pubmed-article:3145093 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3145093 | pubmed:meshHeading | pubmed-meshheading:3145093-... | lld:pubmed |
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| pubmed-article:3145093 | pubmed:meshHeading | pubmed-meshheading:3145093-... | lld:pubmed |
| pubmed-article:3145093 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3145093 | pubmed:articleTitle | Evidence for multiple mechanisms responsible for 2,5-hexanedione-induced neuropathy. | lld:pubmed |
| pubmed-article:3145093 | pubmed:affiliation | Department of Pharmacology, Duke University Medical Center, NC 27710. | lld:pubmed |
| pubmed-article:3145093 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3145093 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
