| pubmed-article:3133355 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0023636 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0051577 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:3133355 | lifeskim:mentions | umls-concept:C2242140 | lld:lifeskim |
| pubmed-article:3133355 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:3133355 | pubmed:dateCreated | 1988-8-4 | lld:pubmed |
| pubmed-article:3133355 | pubmed:abstractText | Expression of the plasmid gene cat-86 is induced in Bacillus subtilis by two antibiotics, chloramphenicol and the nucleoside antibiotic amicetin. We proposed that induction by either drug causes the destabilization of a stem-loop structure in cat-86 mRNA that sequesters the ribosome-binding site for the cat coding sequence. The destabilization event frees the ribosome-binding site, permitting the initiation of translation of cat-86 mRNA. cat-86 induction is due to the stalling of a ribosome in a leader region of cat-86 mRNA, which is located 5' to the RNA stem-loop structure. A stalled ribosome that is active in cat-86 induction has its aminoacyl site occupied by leader codon 6. To test the hypothesis that a leader site 5' to codon 6 permits a ribosome to stall in the presence of an inducing antibiotic, we inserted an extra codon between leader codons 5 and 6. This insertion blocked induction, which was then restored by the deletion of leader codon 6. Thus, induction seems to require the maintenance of a precise spatial relationship between an upstream leader site(s) and leader codon 6. Mutations in the ribosome-binding site for the cat-86 leader, RBS-2, which decreased its strength of binding to 16S rRNA, prevented induction. In contrast, mutations that significantly altered the sequence of RBS-2 but increased its strength of binding to 16S rRNA did not block induction by either chloramphenicol or amicetin. We therefore suspected that the proposed leader site that permitted drug-mediated stalling was located between RBS-2 and leader codon 6. This region of the cat-86 leader contains an eight-nucleotide sequence (conserved region I) that is largely conserved among all known cat leaders. The codon immediately 5' to conserved region I differs, however, between amicetin-inducible and amicetin-noninducible cat genes. In amicetin-inducible cat genes such as cat-86, the codon 5' to conserved region I is a valine codon, GTG. The same codon in amicetin-noninducible cat genes is a lysine codon, either AAA or AAG. When the GTG codon immediately 5' to conserved region I in cat-86 was changed to AAA, amicetin was no longer active in cat-86 induction, but chloramphenicol induction was unaffected by the mutation. The potential role of the GTG codon in amicetin induction is discussed. | lld:pubmed |
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| pubmed-article:3133355 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3133355 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3133355 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3133355 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:3133355 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:3133355 | pubmed:author | pubmed-author:LovettP SPS | lld:pubmed |
| pubmed-article:3133355 | pubmed:author | pubmed-author:DuvallE JEJ | lld:pubmed |
| pubmed-article:3133355 | pubmed:author | pubmed-author:KimU JUJ | lld:pubmed |
| pubmed-article:3133355 | pubmed:author | pubmed-author:AmbulosN... | lld:pubmed |
| pubmed-article:3133355 | pubmed:author | pubmed-author:LortonM AMA | lld:pubmed |
| pubmed-article:3133355 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3133355 | pubmed:volume | 170 | lld:pubmed |
| pubmed-article:3133355 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3133355 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3133355 | pubmed:pagination | 2933-8 | lld:pubmed |
| pubmed-article:3133355 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3133355 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3133355 | pubmed:articleTitle | Site in the cat-86 regulatory leader that permits amicetin to induce expression of the gene. | lld:pubmed |
| pubmed-article:3133355 | pubmed:affiliation | Department of Biological Sciences, University of Maryland Baltimore County, Catonsville 21228. | lld:pubmed |
| pubmed-article:3133355 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3133355 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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