pubmed-article:3120459 | pubmed:abstractText | High-performance liquid chromatography allows one to perform effective and rapid separations on microquantities of material. The miniaturization of the techniques led to new approaches involving isoelectric focusing (IEF) of immobilized pH gradient IEF for haemoglobin preparation, successive enzymic hydrolyses for isolating abnormal peptides and amino acid composition determination after precolumn derivatization. | lld:pubmed |