| pubmed-article:3106354 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C0205103 | lld:lifeskim |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C1511758 | lld:lifeskim |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C1261552 | lld:lifeskim |
| pubmed-article:3106354 | lifeskim:mentions | umls-concept:C1998548 | lld:lifeskim |
| pubmed-article:3106354 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:3106354 | pubmed:dateCreated | 1987-6-5 | lld:pubmed |
| pubmed-article:3106354 | pubmed:abstractText | Our previous studies demonstrated that a site-specific cleavage event initiates the degradation of large premature termination polypeptides of beta-galactosidase in Escherichia coli. We have isolated the first cleavage intermediate, the "B" polypeptide, by elution from sodium dodecyl sulfate-polyacrylamide gels. The NH2 terminus of this protein, determined by automated Edman degradation, was that of the wild-type molecule and thus established that the first cleavage event was at the COOH-terminal end. The sequence of the COOH-terminal end of the B polypeptide was determined by using the enzyme carboxypeptidase Y. Direct assignment of COOH-terminal residues was made by using o-phthaldialdehyde derivatization and the stoichiometry confirmed by a double-label analysis. The COOH-terminal end of the B polypeptide is at position 837 in the beta-galactosidase sequence. If a single endoproteolytic cleavage event was responsible, the cleavage would have occurred between 2 threonine residues (at positions 837 and 838) that are located within a hydrophobic domain. We have observed other covalent modifications that precede the appearance of the B polypeptide, but these do not appear to participate in signaling the first cleavage event. The structure of the COOH-terminal end of B suggests a high degree of specificity by the initial cleavage enzyme. We propose that this unique site serves as a specific signal and that exposure of this site to the specific cleavage enzyme controls the event initiating the degradation pathway. | lld:pubmed |
| pubmed-article:3106354 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3106354 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3106354 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3106354 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3106354 | pubmed:month | May | lld:pubmed |
| pubmed-article:3106354 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3106354 | pubmed:author | pubmed-author:WangS SSS | lld:pubmed |
| pubmed-article:3106354 | pubmed:author | pubmed-author:FriedV AVA | lld:pubmed |
| pubmed-article:3106354 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3106354 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:3106354 | pubmed:volume | 262 | lld:pubmed |
| pubmed-article:3106354 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3106354 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3106354 | pubmed:pagination | 6357-64 | lld:pubmed |
| pubmed-article:3106354 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:meshHeading | pubmed-meshheading:3106354-... | lld:pubmed |
| pubmed-article:3106354 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3106354 | pubmed:articleTitle | Early steps initiating a degradation pathway in Escherichia coli. Characterization of the first intermediate. | lld:pubmed |
| pubmed-article:3106354 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3106354 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3106354 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3106354 | lld:pubmed |
