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pubmed-article:3103373pubmed:abstractTextA polyclonal rabbit antiserum was utilized to localize chondroitin sulfate in human gliomas. Tissue sections were digested with chondroitinase ABC to create the antigenic determinant on the chondroitin sulfate proteoglycan molecule. Normal CNS tissue showed a positive immunohistochemical staining both in white and gray matter, sparing the cytoplasm of glial and neuronal cells. Differentiated astrocytomas presented the same pattern as the normal CNS. Anaplastic astrocytomas and glioblastomas showed progressive reduction of parenchymal positivity as anaplasia increased. These data suggest that chondroitin sulfate is a character expressed by differentiated CNS cells and that it is lost with dedifferentiation. Vascular structures presented positive material in the adventitia in all the oncotypes. A discontinuous positivity was observed in the basal membrane zone of the vessels.lld:pubmed
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pubmed-article:3103373pubmed:dateRevised2007-11-9lld:pubmed
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pubmed-article:3103373pubmed:year1986lld:pubmed
pubmed-article:3103373pubmed:articleTitleImmunohistochemical study of chondroitin sulfate in human gliomas.lld:pubmed
pubmed-article:3103373pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3103373pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed